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接合子细胞强化对生物反应器降解2,4-D效应研究
引用本文:全向春,汤华,马景赟.接合子细胞强化对生物反应器降解2,4-D效应研究[J].环境科学,2011,32(7):2152-2157.
作者姓名:全向春  汤华  马景赟
作者单位:北京师范大学环境学院,水环境模拟国家重点实验室/教育部水沙重点实验室,北京100875
基金项目:国家自然科学基金项目(50878024);国家水体污染控制与治理科技重大专项(2008ZX07209-009-07)
摘    要:研究了接合性质粒pJP4在2株纯菌E.coliDH5α、Alcaligenes sp.及好氧颗粒污泥混合菌群中的水平转移情况,并以获得pJP4质粒的接合子Alcaligenes sp.::pJP4为强化菌种,考察了接合子细胞强化对好氧颗粒反应器和生物膜反应器中难降解有机物2,4-二氯苯氧乙酸(2,4-D)的去除效应.结...

关 键 词:质粒pJP4  基因水平转移  接合子  生物强化  好氧颗粒污泥  生物膜反应器
收稿时间:2010/7/20 0:00:00
修稿时间:2010/8/27 0:00:00

Bioaugmentation of Bioreactors with a pJP4 Receiving Transconjugant to Enhance the Removal of 2,4-D
QUAN Xiang-chun,TANG Hua and MA Jing-yun.Bioaugmentation of Bioreactors with a pJP4 Receiving Transconjugant to Enhance the Removal of 2,4-D[J].Chinese Journal of Environmental Science,2011,32(7):2152-2157.
Authors:QUAN Xiang-chun  TANG Hua and MA Jing-yun
Institution:State Key Laboratory of Water Environment Simulation of Ministry of Education, School of Environment, Beijing Normal University, Beijing 100875, China. xchquan@bnu.edu.cn
Abstract:The paper first investigated horizontal transfer of a conjugative plasmid pJP4 to two pure strains of E. coli DH5alpha and Alcaligenes sp., and a mixed culture of aerobic granular sludge, respectively. With a pJP4 receiving transconjugant Alcaligenes sp. :: pJP4 as the bioaugmented bacteria, bioaugmentation experiments were conducted in an aerobic granular sludge reactor and a biofilm reactor, respectively, to enhance the removal of a recalcitrant compound 2,4-dichlorophenoxyacetic acid(2,4-D). Results showed that pJP4 successfully transferred to E. coli DH5alpha, Alcaligenes sp. and the mixed culture of aerobic granules. For the aerobic granular sludge reactor operated in semi-continuous mode and fed with 2,4-D sole carbon source wastewater, bioaugmentation with Alcaligenes sp. : : pJP4 increased 2,4-D average removal rate significantly with an enhancement of 12% -1 498%. For the biofilm reactor operated in sequence batch mode and fed with mixed carbon sources wastewater, supplementation of the transconjugant reduced system start-up time greatly from 16 d to 5 d. It is a feasible strategy to obtain special degradative transconjugants through gene augmentation and put them into bioreactor as bioaugmentation agent to enhance the removal of some specific pollutants.
Keywords:plasmid pJP4  gene horizontal transfer  transconjugant  bioaugmentation  aerobic granular sludge  biofilm reactor
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