| Responses of Synechocystis sp. PCC 6803 (cyanobacterium) photosystem Ⅱ to pyrene stress |
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| 作者姓名： | Jihai Shao Gongliang Yu Zhongxing Wu Xin Peng Renhui Li |
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| 作者单位： | Jihai Shao(Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;Graduate School of Chinese Academy of Sciences, Beijing 100039, China;College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China);Gongliang Yu,Zhongxing Wu,Xin Peng,Renhui Li(Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China) |
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| 基金项目： | This work was supported by the National Key Science
and Technology Program of China (No. 2008ZX07105-
006), the National Natural Science Foundation of China
(No. 30821140542) and the Hundred Talents Program of
the Chinese Academy of Sciences (No. 0823031501). |
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| 摘 要： |
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| 关 键 词： | photosystem Ⅱ |
| 收稿时间： | 26 September 2009 |
| 修稿时间： | 24 November 2009 |
Responses of Synechocystis sp. PCC 6803 (cyanobacterium) photosystem II
to pyrene stress |
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| Jihai Shao,Gongliang Yu,Zhongxing Wu,Xin Peng,Renhui Li.Responses of Synechocystis sp. PCC 6803 (cyanobacterium) photosystem II
to pyrene stress[J].Journal of Environmental Sciences,2010,22(7):1091-1095. |
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| Authors: | Jihai Shao Gongliang Yu Zhongxing Wu Xin Peng and Renhui Li |
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| Institution: | 1. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;Graduate School of Chinese Academy of Sciences, Beijing 100039, China;College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China
2. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China |
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| Abstract: | In order to explore the mechanism of acute toxicity for pyrene to cyanobacterial organisms, the responses of Synechocystis sp. PCC
6803 photosystem II (PS II) under pyrene stress were studied. The results showed there was no significant di erence about the oxygen
evolution under 0.125 mg/L pyrene stress when compared with control, but it was significantly lower than control at 0.625 mg/L pyrene.
Polyphasic chlorophyll-a fluorescence transients in cells of Synechocystis sp. PCC 6803 exhibited a typical increase including O, J, I,
and P phases. Fluorescence yield at phases J, I and P declined slightly at 0.125 and 0.625 mg/L pyrene, and significantly lower than
control at 3.125 mg/L. According to the parameters deviated from JIP-test, no modification was induced by pyrene both at the donor
side and at the acceptor side of PS II, and the reaction centre of PS II is the primary damaging target. Based on the expressing of four
key genes (psbA, psbB, psbC and psbO) of PS II, only psbA showed significant di erence at 3.125 mg/L pyrene when compared with
control. |
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| Keywords: | pyrene Synechocystis toxicity photosystem II |
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