温瑞塘河沉积物的生物毒性表征研究

利用淡水型发光细菌青海孤菌的一个变种Q67和热带爪蟾胚胎对温瑞塘河沉积物浸出液生物毒性检测分析.沉积物相对发光强度平均值为63.3%,属中等生物毒性,最高发光强度为81.9%,最低发光强度为40.6%.沉积物浸出液的发育毒性结果为胚胎平均孵化率75.98%,最低点为42.5%,最高点为100%;胚胎成活率平均值为83.77%,最低值为66.25%,最高值为100%;平均致畸形率为16.6%,4个采样点无致畸现象,最大致畸性率为36.3%;爪蟾的平均体长为3.58cm,最小体长2.64cm,最大体长4.06cm.工业园区、城市生活与工厂混杂区,河流交汇且易于沉积区域生物毒性大,在源头区及单一生活污染区生物毒性小.

Biotoxicity characterization of sediments from Wenruitang River

The biotoxicity of sediments from Wenruitang River was characterized and analyzed using luminescent bacteria Vibrio qinghaiensis sp. Nov-Q67 and Xenopus tropicalis embryos. The maximum, minimum and average luminous intensity of Q67was 81.9%, 40.6% and 63.3%, respectively, which indicated moderate biotoxicity. In terms of the developmental toxicity of the sediment leachate, the average, maximum and minimum hatching rate of the Xenopus tropicalis embryos was 75.98%, 100% and 42.5%, respectively. The average, minimum and maximum survival rate of the embryos was 83.77%, 66.25% and 100%, respectively. Four sites among all sampling points did not show embryos malformations. The average and maximum teratogenic rate of the embryos was 16.6% and 36.3%. The average, minimum and maximum body length of the embryos was 3.58, 2.64 and 4.06cm, respectively. Sediments collected from combined industrial and residential area exhibited significant biotoxicity, while sediments from the river source area and/or isolated residential area had relatively low biotoxicity.