Changes in metabolic substrates during early development in anchoveta Engraulis ringens (Jenyns 1842) in the Humboldt Current |
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Authors: | M C Krautz S Vásquez L R Castro M González A Llanos-Rivera S Pantoja |
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Institution: | 1. Laboratorio de Oceanografía Pesquera y Ecología Larval (LOPEL), Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, Concepción, Chile 2. Programa de Doctorado en Oceanografía, Departamento de Oceanografía, Universidad de Concepción, Casilla 160-C, Concepción, Chile 3. Instituto de Investigación Pesquera (INPESCA), Casilla 350, Talcahuano, Chile 4. Departamento de Oceanografía y Centro de Investigación Oceanográfica en el Pacífico Sur Oriental (COPAS), Universidad de Concepción, Concepción, Chile 5. Departamento de Bioquímica Clínica e Inmunología, Facultad de Farmacia, Universidad de Concepción, Concepción, Chile 6. Unidad de Biotecnología Marina, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile
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Abstract: | We assessed the ontogenetic changes in protein content and free amino acids (FAA) in eggs and early larvae of Engraulis ringens (anchoveta) off central Chile on different dates during the spawning season. On all sampling dates, a reduction in embryonic
yolk-sac volume, proteins and FAA concentrations occurred during development. Protein electrophoresis (SDS–PAGE) of eggs and
larvae showed at least 22 protein bands: 11 were consumed early and not detected after hatching. The proportion of essential
FAA (EFAA) was higher than the proportion of non-essential FAA (NEFAA) in early eggs and in 7 day-old larvae (82.5-73% EFAA
respectively). During egg development, the FAA pool was dominated by leucine, alanine and lysine, three amino acids contributing
35–44% of the total FAA in eggs. During larval development, histidine was the most abundant FAA. In July, total FAA constituted
13–18% of the egg dry weight. A similar proportion (45–51%) occurred in July between protein plus FAA and total lipids. The
differences in egg size during the spawning season along with variability in batch composition suggests that the female spawning
condition is a major factor determining egg quality and early offspring success. |
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