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氯代邻苯二酚1,2-双加氧酶基因的克隆及其定量表达
引用本文:宋蕾,王慧,施汉昌.氯代邻苯二酚1,2-双加氧酶基因的克隆及其定量表达[J].环境科学,2009,30(2):606-609.
作者姓名:宋蕾  王慧  施汉昌
作者单位:1. 内蒙古工业大学建筑工程学院,呼和浩特,010051
2. 清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084
基金项目:国家重点基础研究发展规划(973)项目(2004CB418506);清华大学基础研究基金项目(JC2003011)
摘    要:

关 键 词:铜绿假单胞菌  氯代邻苯二酚1  2-双加氧酶  1  2  4-三氯苯  实时荧光定量反转录-PCR
收稿时间:1/9/2008 12:00:00 AM
修稿时间:1/9/2008 12:00:00 AM

Cloning and Quantitative Expression of Chlorocatechol 1,2-Dioxygenase Gene
SONG Lei,WANG Hui and SHI Han-chang.Cloning and Quantitative Expression of Chlorocatechol 1,2-Dioxygenase Gene[J].Chinese Journal of Environmental Science,2009,30(2):606-609.
Authors:SONG Lei  WANG Hui and SHI Han-chang
Institution:1.College of Architecture and Civil Engineering;Inner Mongolia University of Technology;Huhhot 010051;China;2.State Key Joint Laboratory of Environmental Simulation and Pollution Control;Department of Environmental Science and Engineering;Tsinghua University;Beijing 100084;China
Abstract:The chlorocatechol 1,2-dioxygenase gene,designated as tcbC(J5-2)(GenBank Accession No.EF111021),was cloned from total DNA of Pseudomonas aeruginosa J5-2 which can degrade 1,2,4-trichlorobenzene.The deduced amino acid sequence analysis of tcbC(J5-2)shows that it is different from tcbC(P51)cloned from another 1,2,4-trichlorobenzene-degrader Pseudomonas sp.P51.tcbC(J5-2)is only 95% similar to tcbC(P51)at the amino acid level.Three critical amino acid residues in tcbC(J5-1)are Leu-48,Ala-52,and Ile-73.Otherwise...
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