| 氯代邻苯二酚1，2-双加氧酶基因的克隆及其定量表达 |
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| 引用本文： | 宋蕾,王慧,施汉昌. 氯代邻苯二酚1，2-双加氧酶基因的克隆及其定量表达[J]. 环境科学, 2009, 30(2): 606-609 |
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| 作者姓名： | 宋蕾 王慧 施汉昌 |
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| 作者单位： | 1. 内蒙古工业大学建筑工程学院,呼和浩特,010051
2. 清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084 |
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| 基金项目： | 国家重点基础研究发展规划(973)项目(2004CB418506)；清华大学基础研究基金项目(JC2003011) |
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| 摘 要： |
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| 关 键 词： | 铜绿假单胞菌 氯代邻苯二酚1,2-双加氧酶 1,2,4-三氯苯 实时荧光定量反转录-PCR |
| 收稿时间： | 2008-01-09 |
| 修稿时间： | 2008-01-09 |
Cloning and Quantitative Expression of Chlorocatechol 1,2-Dioxygenase Gene |
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| SONG Lei,WANG Hui and SHI Han-chang. Cloning and Quantitative Expression of Chlorocatechol 1,2-Dioxygenase Gene[J]. Chinese Journal of Environmental Science, 2009, 30(2): 606-609 |
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| Authors: | SONG Lei WANG Hui SHI Han-chang |
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| Affiliation: | 1.College of Architecture and Civil Engineering;Inner Mongolia University of Technology;Huhhot 010051;China;2.State Key Joint Laboratory of Environmental Simulation and Pollution Control;Department of Environmental Science and Engineering;Tsinghua University;Beijing 100084;China |
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| Abstract: | The chlorocatechol 1,2-dioxygenase gene,designated as tcbC(J5-2)(GenBank Accession No.EF111021),was cloned from total DNA of Pseudomonas aeruginosa J5-2 which can degrade 1,2,4-trichlorobenzene.The deduced amino acid sequence analysis of tcbC(J5-2)shows that it is different from tcbC(P51)cloned from another 1,2,4-trichlorobenzene-degrader Pseudomonas sp.P51.tcbC(J5-2)is only 95% similar to tcbC(P51)at the amino acid level.Three critical amino acid residues in tcbC(J5-1)are Leu-48,Ala-52,and Ile-73.Otherwise... |
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