Effects of copper on spore germination, growth and ultrastructure of Polypodium cambricum L. gametophytes |
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Authors: | Muccifora S |
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Institution: | Dipartimento di Biologia Evolutiva, Università di Siena, via A. Moro 4, 53100 Siena, Italy. muccifora@unisi.it |
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Abstract: | The effects of different concentrations (10(-5), 5x10(-5) and 10(-4)M) of copper bromide on spore germination, growth and ultrastructure were investigated in Polypodium cambricum L. gametophytes. The inhibitory effect of Cu was observed in spores cultured on medium supplemented with 10(-4)M CuBr(2): germination occurred about 40 days after sowing and was only 25%. Concentrations of 5x10(-5) and 10(-4)M CuBr(2) induced changes in gametophyte development, possibly by re-orientation of growth. Gametophytes treated with 10(-5) and 5x10(-5)M CuBr(2) took up and accumulated a large amount of copper and ultrastructural observations showed that cytoplasmic damage was limited to twisted swollen thylakoids. The ultrastructure of gametophytes treated with 10(-4)M CuBr(2) showed absence of a vacuolar compartment. The present observations suggest that P. cambricum gametophytes could be a suitable material for studying physiological and molecular alterations induced by excess copper. |
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Keywords: | Polypodium cambricum Gametophyte Copper TEM |
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