| 铀和铅对枯草杆菌毒性的研究 |
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| 引用本文： | 刘清,徐伟昌,招国栋,赵由才.铀和铅对枯草杆菌毒性的研究[J].环境污染与防治,2007,29(4):247-249,253. |
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| 作者姓名： | 刘清 徐伟昌 招国栋 赵由才 |
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| 作者单位： | 1. 同济大学污染控制与资源化研究国家重点实验室,上海,200092
2. 南华大学建筑工程与资源环境学院,湖南,衡阳,421001 |
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| 摘 要： | 采用3种方法研究了铀和铅对枯草杆菌的毒性:(1)试纸法测抑菌圈的直径;(2)血球计数板测细菌菌数;(3)高倍显微镜下观察细菌形态和密度的变化.实验考察铀和铅的质量浓度为1～2 000 mg/L.结果表明:铀和铅对枯草杆菌的生长繁殖有显著影响;铀和铅对枯草杆菌的最大无作用剂量均小于1 mg/L;铀对枯草杆菌的致死质量浓度为500 mg/L,铅对枯草杆菌的致死质量浓度为 1 000 mg/L.同时,对以上3种方法进行了比较,确定了其各自的应用范围,为重金属对微生物毒性研究方法的选择提供了参考.
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| 关 键 词： | 毒性 枯草杆菌 铀 铅 枯草杆菌 生物毒性 研究方法 lead uranium effects 的选择 对微 重金属 范围 应用 比较 最大无作用剂量 影响 生长繁殖 结果 质量浓度 考察 实验 变化 |
| 修稿时间： | 2006-05-22 |
Toxic effects of uranium and lead to B. subtilis |
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| Liu Qing,Xu Weichang,Zhao Guodong,Zhao Youcai.Toxic effects of uranium and lead to B. subtilis[J].Environmental Pollution & Control,2007,29(4):247-249,253. |
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| Authors: | Liu Qing Xu Weichang Zhao Guodong Zhao Youcai |
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| Institution: | 1. StateKey Laboratory of Pollution Control and Resources Reuse Research, Tongji University, Shanghai 200092,2. School of Architectural Engineering, Resources and Environment, Nanhua University, Hengyang Hunan 421001 |
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| Abstract: | The toxic effects of uranium and lead to B. subtilis were evaluated based on three quantitative parameters: diameter of the circle of bacteriostasis, bacterial population measured by Heamacytometer, and changes in shape and density of the bacteria. Both uranium and lead were found to inhibit the growth of B. subtilis at 1 mg/L or lower;the lethal concentration of U and Pb to B. subtilis was 500 mg/L and 1 000 mg/L,respectively. The change in bacterial population was the most sensitive of the three parameters as a simple indicator of the metals' toxic effects. |
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| Keywords: | Toxic effects B subtilis Uranium Lead |
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