硫酸盐对Nitrate-DAMO反应过程影响及动力学特性

反硝化型甲烷厌氧氧化（Nitrate-/nitrite-denitrifying methane anaerobic oxidation，N-DAMO）技术是一种新型的同步脱氮除甲烷技术.我国环境水体和污水中普遍存在硫酸盐，为探明不同浓度硫酸盐对N-DAMO过程的影响，本研究通过短期和长期试验考察了硫酸盐对Nitrate-DAMO系统生物反应过程的影响，并分析了该过程的动力学及化学计量学特性的影响规律.结果表明，不同浓度梯度的硫酸盐（0~3.96 mmol·L^-1）对Nitrate-DAMO系统的短期影响呈先促进后抑制趋势，在浓度为0.42 mmol·L^-1时促进作用最明显；当浓度达到0.83 mmol·L^-1时，开始有明显的抑制作用，且长期和短期试验过程中均未出现硫酸盐消耗现象.0~0.42 mmol·L^-1和0.42~0.83 mmol·L^-1硫酸盐对Nitrate-DAMO系统脱氮性能影响的动力学过程分别符合非底物抑制方程和Haldane方程，通过拟合得出硫酸盐对Nitrate-DAMO系统的初始抑制浓度和令Nitrate-DAMO系统完全失去脱氮性能的硫酸盐浓度分别为0.81和4.06 mmol·L^-1.硫酸盐对Nitrate-DAMO系统甲烷氧化速率影响的动力学过程符合非底物抑制方程，实际的甲烷氧化速率与脱氮速率之比（5.03：8）符合理论值（5：8）.同时，硫酸盐还会影响N-DAMO微生物的生长周期.

The performance and kinetic characteristics of nitrate-denitrifying anaerobic methane oxidation process under different sulfate concentration

N-DAMO (nitrate-/nitrite-denitrifying methane anaerobic oxidation) technology can achieve nitrogen removal and methane reduction simultaneously. Short- and long-term experiments were carried out to find out the effect of sulfate on N-DAMO process, and the dynamics and stoichiometry of the process were also analyzed. Experimental results indicated that the performance of the Nitrate-DAMO system was promoted firstly then inhibited with the increase of SO₄^2- concentration within 3.96 mmol·L^-1, and the denitrification rate was highest at 0.42 mmol·L^-1. There was no sulfate consumed during the whole experiment. The denitrification kinetics conformed to non-substrate inhibition equation and Haldane equation. The initial inhibitory and completely inhibited concentration of sulfate on the Nitrate-DAMO system was 0.81 and 4.06 mmol·L^-1, respectively. The sulfate effect on methane oxidation rate in the Nitrate-DAMO system was consistent with the non-substrate inhibition equation, and the ratio of the actual methane oxidation rate to the nitrogen removal rate (5.03:8) is in line with the theoretical value (5:8). In addition, sulfate can influence the growth cycle of N-DAMO microorganisms in the Nitrate-DAMO system.