全氟辛烷磺酸盐(PFOS)对半滑舌鳎(Cynoglossus semilaevis)免疫相关基因表达的影响

为了解全氟辛烷磺酸盐(perfluorooctane sulfonate, PFOS)暴露对半滑舌鳎(Cynoglossus semilaevis)免疫功能的影响,在实验室条件下,运用RT-PCR方法分析了PFOS暴露对半滑舌鳎热休克蛋白hsp70、hsp90、C型凝集素(c-type lectin)和细胞色素c氧化酶(cytochrome c oxidase, cox)等4种免疫相关基因表达水平的影响。实验测定了上述4种基因在半滑舌鳎肝、鳃、肠及肌肉4种不同组织中随时间(0、24 h、48 h、96 h和7 d)的表达变化情况。结果表明,在4种组织中,hsp70基因的表达与对照相比为上调,其中,肝组织hsp70基因的表达量显著高于其他各组织,且表达高峰值的出现也早于其他各组织;hsp90基因在肝和鳃组织中表达量随时间不同而波动,在肠组织中表达上调,在肌肉中表达显著下调;c-type lectin基因表达量与对照组相比表达显著下调或无明显差异;cox基因在肝组织和肠组织中表达下调,在鳃和肌肉中表达上调。上述研究结果表明,PFOS能引起免疫相关基因的表达变化,对半滑舌鳎具有潜在的免疫毒性。肝组织中各免疫基因对PFOS胁迫的响应高于其他组织。本研究可为阐明全氟辛烷磺酸盐对半滑舌鳎的免疫毒性提供基础数据。

Effects of Perfluorooctane Sulfonate (PFOS) on Immune-related Gene Expression in Half-smooth Tongue Sole (Cynoglossus semilaevis)

Perfluorooctane sulfonate (PFOS) is a persistent organic pollutant. Due to the extensive use of its products, PFOS enters the environment and organisms in various ways and accumulates through the food chain. To understand the effect of PFOS on half-smooth tongue sole (Cynoglossus semilaevis) immune function, we treated 6-month older fish with 2 mg L^?1 PFOS for 0, 24 h, 48 h, 96 h, and 7 d, respectively. The expressions of four immune-related genes, including the heat shock protein (hsp70, hsp90), c-type lectin domain (c-type lectin), and cytochrome c oxidase (cox), were analyzed in the liver, gill, intestine, and muscle tissues under different exposure time. The results showed that the expression of hsp70 in these four tissues was significantly up-regulated under exposure respectively. Meanwhile, its expression in liver was significantly higher than other tissues, and the peaked expression of hsp70 in liver appeared after 24 h PFOS exposure, which responded much earlier than other tissues. The expression of hsp90 increased significantly after 48 h exposure, recovered to normal after 7 d exposure in liver and gill, and was up-regulated in intestine and down-regulated in muscle significantly. The expression of c-type lectin was significantly down-regulated or not significantly different compared with the control in four tissues. The expression of cox was significantly up-regulated in liver and intestine, down-regulated in gill and muscle. These results indicated that different tissues in half smooth tongue sole have different immune reaction in regards of PFOS treatment, which has a potential immunotoxicity to half-smooth tongue sole. When faced with PFOS stress, the immune-related genes in the liver could response more quickly. These results could be helpful to understand the immunotoxicity of PFOS on half-smooth tongue sole.