不同短程硝化系统中微生物群落结构的对比分析

为探讨有机碳源对短程硝化系统中微生物群落结构的影响,采用构建克隆文库的方法对模拟无机城市生活污水和模拟实际城市生活污水短程硝化系统中的微生物群落结构进行对比分析.实验结果表明,变形菌门(Proteobacteria)和未培养菌(uncultured bacterium)是两系统中的优势菌群.两系统中的菌群结构存在差异,但优势菌群及其所占比例相似.两系统中的主要脱氮菌类群也相似,但由于有机碳源浓度的不同其菌属及比例有所差异.无机短程硝化系统中的脱氮菌群包括亚硝化单胞菌属(Nitrosomonas)、硝化螺菌属(Nitrospira)和Denitratisoma,其中自养硝化菌的比例高于其在有机短程硝化系统中的比例,但仍低于异养菌在该系统中的比例.有机短程硝化系统中的脱氮菌群主要包括β-Proteobacteria中的一些反硝化细菌和亚硝化单胞菌属(Nitrosomonas),其中亚硝化单胞菌属(Nitrosomonas)的含量很少.

Analysis of microbial community structure in different partial nitrification system

In order to study the influences of organic carbon source on the microbial community structure in partial nitrification system, the microbial diversity was investigated in two partial nitrification reactors by the construction of bacterial clone library. The influents of two reactors were artificial wastewater with and without organic carbon source, respectively. The results indicated that Proteobacteria and uncultured bacterium were the dominant bacteria of the two studied systems. The differences existed in the structure of the bacterial community of the two systems. Nonetheless, the dominant bacteria and their proportions were similar to each other in the total clones. In addition, the main denitrifer groups were also similar in the two systems. Due to the different concentrations of organic carbon source, the denitrifer genera and their proportions were distinct. The main denitrifer genera of partial nitrification reactor with inorganic wastewater were Nitrosomonas, Nitrospira and Denitratisoma. The proportion of autotrophic nitrifying bacteria in partial nitrification reactor with inorganic wastewater was higher than that with organic wastewater. But in the reactor with inorganic wastewater, the proportion of autotrophic bacteria was lower than heterotrophic bacteria. The main denitrifer genera of partial nitrification reactor with organic wastewater were some denitrifying bacteria of β-Proteobacteria and Nitrosomonas. Additionally, the proportion of the latter in this reactor was relatively low.