| 筛选菌种酶催化降解微囊藻毒素的特点 |
| |
| 引用本文： | 何宏胜,闫海,周洁,葛世友,肖宝清,吕乐.筛选菌种酶催化降解微囊藻毒素的特点[J].环境科学,2006,27(6):1171-1175. |
| |
| 作者姓名： | 何宏胜 闫海 周洁 葛世友 肖宝清 吕乐 |
| |
| 作者单位： | 北京科技大学应用科学学院生物科学与技术系,北京,100083;北京科技大学土木与环境工程学院环境工程系,北京,100083;北京科技大学应用科学学院生物科学与技术系,北京,100083;北京科技大学土木与环境工程学院环境工程系,北京,100083 |
| |
| 基金项目： | 国家自然科学基金项目(30270277,20377047);北京市自然科学基金项目(8063029);中国科学院微生物研究所微生物资源国家重点实验室开放基金项目(031029) |
| |
| 摘 要： | 从云南滇池底泥中筛选出1株能够高效降解微囊藻毒素(Microcystins,MCs)的纯菌种.该菌无细胞提取液能进行降解MC-RR和MC-LR的酶促反应,并有最终产物的积累.在pH 6～8范围内酶对MC-RR和MC-LR的催化降解活性较高,Cu2+、Mn2+和Zn2+对降解MCs的酶促反应没有明显的促进作用.
|
| 关 键 词： | 微囊藻毒素 生物降解 酶促反应 |
| 文章编号： | 0250-3301（2006）06-1171-05 |
| 收稿时间： | 2005-06-20 |
| 修稿时间： | 2005-06-202005-08-29 |
Characterization of Enzymatic Degradation of Microcystins by a New Isolated Bacterium |
| |
| He Hong-sheng,Yan Hai,Zhou Jie,Ge Shi-you,Xiao Bao-qing,Lü Le.Characterization of Enzymatic Degradation of Microcystins by a New Isolated Bacterium[J].Chinese Journal of Environmental Science,2006,27(6):1171-1175. |
| |
| Authors: | He Hong-sheng Yan Hai Zhou Jie Ge Shi-you Xiao Bao-qing Lü Le |
| |
| Institution: | 1. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China; 2.Department of Environmental Engineering, School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China |
| |
| Abstract: | A strain of bacterium capable of biodegrading microcystin(MC) RR and MC-LR was isolated from the sediments of Dianchi Lake.It was demonstrated that the enzymes in cell-free extract of this bacterium were responsible for the degradation of MC-RR and MC-LR,and the dead-end products of MC-RR and MC-LR catalyzed by these enzymes were observed on HPLC chromatograms.Results show that the optimum pH for the activities of these enzymes was in the range from 6.0 to 8.0,however the metal ions of Cu2+,Mn2+ and Zn2+ had no apparent effects on the enzymatic degradation of MC-RR and MC-LR. |
| |
| Keywords: | microcystins biodegradation enzymatic degradation |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
| 点击此处可从《环境科学》浏览原始摘要信息 |
| 点击此处可从《环境科学》下载免费的PDF全文 |
|
