1,2,4-三氯苯对大蒜根尖分生细胞有丝分裂的影响

以大蒜根尖分生细胞为材料研究1,2,4-三氯苯(1,2,4-TCB)对有丝分裂的影响.结果表明,1,2,4-TCB使大蒜根尖分生细胞有丝分裂指数下降,且呈现明显的剂量-效应和时间-效应关系;1,2,4-TCB使有丝分裂前期细胞所占比例相对增加,中期、后期、末期细胞相对减少;与后期、末期相比,中期细胞的减幅更大;1,2,4-TCB各处理组的微核细胞发生率与对照组相比均没有统计学差异;双核细胞发生率在高浓度处理组与对照组相比,有极显著性的统计学差异(p＜0.01),并呈现一定的剂量-效应和时间-效应关系.研究表明,1,2,4-TCB对大蒜根尖分生细胞有丝分裂具有阻滞作用,并诱发有丝分裂异常的显著升高.

Effects of 1,2,4-trichlorobenzene on the mitosis in root meristematic cells of A. sativum seedlings

The damage effects of 1,2,4-trichlorobenzene on the mitosis in root meristematic cells of A. sativum seedlings were studied. The results showed the mitotic index in root meristematic cells of A. sativum seedlings were decreased in treated time-response and dose-response manners. The prophase cell ratios among the mitotic cells were relatively increased, while the metaphase, anaphase and telaphase cells ratios were decreased; Compared with anaphase and telophase cells, the decreasing amplitude of metaphase cells was bigger. Compared to the control groups, the frequencies of micronuclei in 1,2,4-trichlorobenzene groups showed no statistic difference, whereas the frequencies of binucleated cells showed distinguished statistic difference (p< 0.01). The increasing of frequency of binucleated cells was dependent on treated time and concentrations. Conclusion: The mitosis in root meristematic cells of A. sativum seedlings were obviously changed after the treatment of 1,2,4-trichlorobenzene, and the abnormal division were increased.