Mn2+促进载体挂膜的机理研究

通过向自制固定床生物膜反应器(FBBR)中投加氯化锰,考察了Mn~(2+)对生物膜量、形态结构、降解性能及其胞外聚合物(EPS)组分的影响,并探究了Mn~(2+)在生物膜形成中的作用、影响规律及其对生物膜反应器运行效能的影响.结果表明,投加Mn~(2+)有助于生物膜形成,增强生物膜致密性,并促进生物膜生长;在生物膜成熟期连续投加10 mg·L~(-1)Mn~(2+)对一个周期内生物膜的降解活性影响很小,反应器COD和NH_4~+去除率分别达到85.4%±1.5%和76.3%±1.9%;生物膜不同类型EPS含量的变化表明,Mn~(2+)不仅加速了生物膜的成熟和增强了生物膜去除有机物的稳定性,而且还会刺激生物膜分泌产生更多的紧密粘附型EPS,其中的蛋白质和多糖能够保护微生物免受Mn~(2+)伤害.

The promotion mechanism of Mn2+ for biofilm formation on carrier

The effects of Mn²⁺ on the biomass, morphological structure, degradation performance and extracellular polymeric substance (EPS) of biofilm were investigated by dosing manganese chloride in the self-made fixed bed biofilm reactors (FBBRs). Meantime, the operational performance of biofilm reactor has also been studied. Results show Mn²⁺ amendment contributed to the biofilm formation, and the compactness of the biofilm structure during the biofilm development. Continuously dosing 10 mg·L^-1 of Mn²⁺ showed little effect on the removal of both organic matter and ammonia nitrogen, which reached 85.4%±1.5% and 76.3%±1.9% respectively in an operating cycle in the biofilm maturation period. Furthermore, with the different types of EPS contents, the Mn²⁺ not only promoted the biofilm maturation also enhanced the stability of the organic matter removal. The stimulated production of TB-EPS, including protein and polysaccharide, both of which can protect microorganisms against the adverse influences caused by Mn²⁺.