The use of polymerase chain reaction for the identification of sciaenid eggs |
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Authors: | Lucia B Carreon-Martinez Scott A Holt B Scott Nunez Cynthia K Faulk G Joan Holt |
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Institution: | (1) Great Lakes Institute for Environmental Research, University of Windsor, Windsor, ON, Canada;(2) The University of Texas at Austin Marine Science Institute, Port Aransas, TX, USA;(3) Lee College, Baytown, TX, USA |
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Abstract: | Traditionally, sciaenid eggs have been identified based on morphological characteristics such as size, number of oil globules
and/or pigmentation patterns. Identification of sciaenid eggs by these procedures is time consuming and often inaccurate due
to considerable egg size overlap among species. The utilization of molecular techniques for the identification of economically
important species has become a fundamental component in ecological studies involving fish eggs and larvae. This study reports
the development of a series of both multiplex and individual polymerase chain reactions to identify the eggs of 11 sciaenid
species commonly found in the Gulf of Mexico and estuaries near Port Aransas and Corpus Christi, TX, USA. Following method
development, the discriminatory power of the assay was first determined with samples from adult fish collected from Aransas
and Corpus Christi Bays, Galveston Bay and the lower Laguna Madre in northern Mexico. Most (97%) of these fishes were correctly
identified to the level of species. To demonstrate the applicability of the assay, wild fish eggs were collected and analyzed
from the Aransas Pass tidal inlet from September through December 2005. During this period, the eggs of four target species
were positively identified which was in keeping with current knowledge regarding the spawning areas and seasons of these sciaenids
based on the presence of mature females, eggs and/or larvae. Future use of this method can provide valuable information to
better discriminate spawning sites and seasons of these species. |
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