|
|||||
|
|
| 二价铜离子与抗坏血酸共存下的杀菌效果及其机理 | |
| 引用本文： | 张策,庄惠生,柿井一男.二价铜离子与抗坏血酸共存下的杀菌效果及其机理[J].环保科技,2010,16(2):31-36,45. |
| 作者姓名： | 张策 庄惠生 柿井一男 |
| 作者单位： | 1. 东华大学环境科学与工程学院,上海,201620 2. 东华大学环境科学与工程学院,上海,201620;上海交通大学环境科学与工程学院,上海,200240 3. 日本宇都宫大学工学部,日本宇都宫,321-0912 |
| 基金项目： | 上海市科技攻关项目，上海市重点学科建设项目 |
| 摘 要： | 考察了二价铜离子与抗坏血酸的组合对大肠杆菌的杀菌效果。实验证明:高浓度的铜离子与抗坏血酸的组合有非常好的杀菌效果,不论是酸性还是中性条件下都能在0.5 h杀灭所有的大肠杆菌;低浓度的铜离子与抗坏血酸的组合也有不错的杀菌效果,且随着pH值的升高,杀菌效果增强,pH=4的条件下24 h后杀灭大肠杆菌,pH=7的条件下0.5 h便能杀灭所有杆菌。实验运用ESR检测羟基自由基,并结合杀菌实验结果推断二价铜离子与抗坏血酸反生了类Fenton反应。 |
| 关 键 词： | 铜离子 抗坏血酸 ESR 羟基自由基 类Fenton反应 |
Bactericidal Effects and Mechanism of L-Ascorbic Acid plus Cu2+ on Escherichia Coli Cells |
|
| Authors: | ZHANG Ce ZHUANG Huisheng Kakii Kazuo |
| Institution: | ZHANG Ce, ZHUANG Huisheng , Kakii Kazuo (1. School of Environmental Science and Engineering, Donghua University, Shanghai 201620, China; 2. School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China ; 3. Department of Engineering of Utsunomiya University, Utsunomiya 321 - 0912, Japan) |
| Abstract: | This paper studied the bactericidal effects of L - Ascorbic Acid （ L - AA） plus Cu2＋ on Escherichia coli ceils. The results of experiments showed that the system of Cu2＋ with high concentration plus L - AA had excellent bactericidal effect, all cell - killing could be reached after half an hour no matter at acidic or neutral conditions. The system of Cu2＋ with low concentration plus L - AA also had no bad bactericidal effect, and the cell - killing rate was increased when pH raised, the time needed for all cell - killing at pH 4 and pH 7 was 24 h and 0.5 h, respectively. ESR was applied to detect hydroxyl radicals, combining with the results of bactericidal experiments, it could draw that a Fenton -like reaction had taken place between Cu2＋ and L- AA. |
| Keywords: | ESR |
| 本文献已被 维普 万方数据 等数据库收录！ | |