海藻酸钠固定石油降解菌条件优化及辅助效应

随着海洋石油污染治理技术的发展，生物修复技术因为具有成本低、效率高、环境友好的特点备受关注，而固菌技术可提高生物修复中石油烃降解菌的降解效率。本次研究利用海藻酸钠和CaCl₂对石油烃降解菌进行固定成球试验，并利用四因素三水平正交试验对固菌过程的条件参数（如:海藻酸钠浓度、菌悬液添加量、CaCl₂浓度和交联时间）进行优化。结果表明:采用固菌方式可有效提高石油烃降解菌的降解效率，当海藻酸钠浓度为3%，菌悬液添加量75%，CaCl₂浓度3%时，交联时间30 h时达到最优固定化效果，石油烃降解菌微球7 d绝对降解率为48.7%，比直接添加菌剂提高28%。

Optimization and auxiliary effects of sodium alginate immobilized petroleum degrading bacteria

Along with the develop of Marine oil pollution treatment technology, the biological treatment technology have got much attention because it is a cost-effective, highly active and environmentally friendly method, among which the immobilization of strain can improve the degrading efficiency of oil degrading bacteria in bioremediation process.In the study, sodium alginate (SA) -calcium chloride were used to immobilize the oil degrading bacteria into microspheres, and the four-factor orthogonal test at three levels was carried out to optimize the parameters of the bacteria immobilization process which includes the concentration of sodium alginate, the bacterial suspension dosage, the concentration of calcium chloride and the cross-linking time).The result showed that the best immobilized condition was sodium alginate concentration 3%, bacterial suspension dosage 75%, calcium chloride concentration 3% and cross-linking time 30 h.Under these conditions, the 7 d absolute degradation rate of oil degrading bacteria microsphere was 48.7%, which was 28% higher than CK.