黄曲霉A5p1对偶氮染料的降解特性和降解途径

以黄曲霉菌株A5p1为生物材料，研究其脱色染料的广谱性，并选择偶氮染料直接蓝71（DB71）为模型底物，探讨脱色特性及降解产物.该菌株对15种染料的脱色测试结果表明，染料浓度为100mg/L时脱色效率为61.7%~100%.该菌对偶氮染料DB71具有生物吸附和生物降解的双重作用，在pH值7.0，温度30℃，染料浓度300mg/L，蔗糖为碳源时对DB71 脱色率为100%.酶分析显示葡萄糖氧化酶和锰过氧化物酶参与染料的降解.FTIR、GC-MS和LC-MS分析确定代谢终产物为萘胺、叠氮萘、2-羟基-6-草酰-苯甲酸和1-萘酚.

Decolorization exploration of azo dye by Aspergillus flavus A5p1: characteristics and biodegradation pathway

Aspergillus flavus strain A5p1 was used as a biological material to study its broad decolorization spectrum and decolorization characteristics and degradation products with the azo dye Direct Blue 71 (DB71) as the model substrate. The results showed that the decolorization efficiency of 15 kinds of dyes ranged from 61.7% to 100.0% with the 100mg/L dye concentration. There might be a synergistic effect of biosorption and biodegradation on the decolorization. The optimum decolorization condition of A5p1 for azo dye DB71 was pH 7.0, temperature 30℃, dye concentration 300mg/L, and sucrose as a carbon source. Enzyme analysis revealed that glucose oxidase and manganese peroxidases were involved in the degradation process. FTIR, GC-MS and LC-MS analyses indicated that the intermediates of azo dye degradation included naphthalene amine, naphthalene diazonium, 2-hydroxy-6-oxal-yl-benzoic acid and 1-naphthol.