异养菌与新型填料成膜性及BTF处理屠宰H2S废气

以新型填料上生物膜的微生物种群结构为研究对象，将异养脱硫细菌蜡状芽孢杆菌ZJNB-B3接种到含活性污泥和LEVAPOR新型填料的小试曝气液体反应器，研究此菌在活性污泥及在填料生物膜中的微生物多样性.在属分类水平上的物种分析结果表明，芽孢杆菌属在接种后的填料中相对丰度较高，而在活性污泥样品中相对丰度较低，其在培养了第10d的填料样品中已经达到最大相对丰度.因此该菌与新型填料的亲和性和成膜性较好.将该菌与新型填料应用于生物滴滤塔（BTF）处理屠宰污水站排放的H₂S恶臭废气，风量处理能力为2000m³/h.结果表明，经该菌强化的BTF完成启动的时间比使用普通活性污泥接种的BTF缩短7d.强化的BTF在30d的稳定期试验中，当H₂S进气浓度为4.92~9.54mg/m³时，对H₂S去除率为98%~99%.当进气H₂S在1.0~10mg/m³范围波动时，空床停留时间（EBRT）为10，21，30s时，去除率分别为94%、98%、99%以上，且受进气H₂S浓度波动的影响不大.当进口负荷为0.60~1.14g/（m³·h）时，H₂S去除率稳定在98%以上，H₂S气体排放量低于恶臭污染物排放国家标准.

Biofilm formation of a heterotrophic bacterium on the novel packing material and the removal of hydrogen sulfide produced in a slaughtering process by biotrickling filter

The microbial taxonomic composition distribution of biofilm on the novel packing material was studied. The heterotrophic desulfurization bacterium Bacillus cereus ZJNB-B3 was inoculated and injected into an aeration liquid reactor containing activated sludge and LEVAPOR novel packing material. The microbial diversity of the bacteria in the reactor was evaluated. The taxonomic composition distribution of Genus-level showed that Bacillus had a higher relative abundance on the LEVAPOR packing material while lower in activated sludge. On the 10th day of the aeration reaction, the maximum relative abundance of the Bacillus on the LEVAPOR packing samples was achieved. Therefore, the Bacillus cereus ZJNB-B3 strain has excellent affinity with LEVAPOR packing material. Afterward, the removal of hydrogen sulfide of slaughtering sewage by biotrickling filter (BTF) inoculated with the desulfurization bacterium was investigated. The treatment capacity was 2000m³/h. The start-up period of the BTF inoculated with the desulfurization bacterium was 7days, which was 7days shorter than that of the BTF only inoculated with activated sludge. The stable operation of the enhanced BTF was carried out for 30days. When the inlet concentration of H₂S was 4.92~9.54mg/m³, the removal efficiency (RE) for H₂S was achieved by 98%~99%. When the empty bed residence time (EBRT) were 10s, 21s, and 30s, the RE was above 94%, 98% and 99% respectively. And the inlet H₂S concentration of 1.0~10mg/m³ had no obvious influence on the RE. When the inlet load was 0.60~1.14g/(m³·h), the RE was above 98%. The emission of H₂S was lower than the national emission standards for odor pollutants.