好氧颗粒污泥形成过程中群感效应的作用研究

通过观察污泥颗粒化过程中胞外聚合物和水解氨基酸的变化情况，同时监测在好氧颗粒污泥形成过程中不同种类酰化高丝氨酸内酯类化合物（AHLs）在AGS形成过程的变化规律，协同研究群感效应对胞外聚合物产生的影响，进而揭示颗粒化过程中污泥变化特征.结果表明，絮状污泥颗粒化过程中，AGS最终平均粒径约为150 μm，胞外聚合物中蛋白质增幅较大且以紧密结合型胞外聚合物及Pellet层胞外聚合物增加为主.污泥胞外聚合物中芳香族类物质及酪氨酸蛋白物质的生成是AGS形成的物质基础；丙氨酸、甲硫氨酸等疏水性物质可促进AGS的生成并维持其稳定.在AGS形成过程中，群感效应整体呈现为加强趋势，其中C4-HSL呈现明显浓度下降趋势，C6-HSL，3OC6-HSL及C8-HSL与胞外聚合物，蛋白质及多糖均呈现正相关趋势，其中C6-HSL和C8-HSL与紧密结合型胞外聚合物中的蛋白质及多糖呈现极显著正相关.

Effect of quorum sensing in the formation of aerobic granular sludge

By observing the variations of EPS, hydrolytic amino acids and acyl-homoserine lactones (AHLs) in the formation of aerobic granular sludge (AGS), the effect of quorum sensing is discovered in depth. The experimental results showed that in the granulation process, the final average size of AGS was approximately 150μm and the protein in EPS increased significantly, and the tightly bound EPS (TB-EPS) played an important role for granulation. The formation of aromatic substances and tyrosine protein substances in EPS provides material basis for AGS formation, and hydrophobic substances alanine and methionine promotes the formation of AGS and maintains the stability. Quorum sensing was generally strengthened during the formation process of AGS. The concentration of C4-HSL decreased significantly, while C6-HSL, 3OC6-HSL and C8-HSL were correlated with EPS, PN and PS, furthermore, C6-HSL and C8-HSL were presented significantly positively correlated with proteins and polysaccharides in TB-EPS.