螯合型表面活性剂强化黑麦草修复Cd污染水体

采用水培试验，研究了新型螯合型表面活性剂N-十二酰基乙二胺三乙酸钠（LED3A）强化黑麦草修复Cd污染水体的效果，探讨了Cd或/和LED3A存在下黑麦草的生理响应以及LED3A对黑麦草除Cd性能的影响.结果表明：Cd显著抑制黑麦草生物量和光合色素含量，添加LED3A后抑制作用略有增强，CL100处理叶绿素和类胡萝卜素含量较CL0分别降低了15.50%和8.18%. LED3A对黑麦草的生长无显著影响，对黑麦草细胞的相对电导率（REC）有一定的提高，可使细胞膜渗透性增加以利于黑麦草对Cd的吸收.Cd胁迫下适量添加LED3A，能提高黑麦草植株超氧化歧化酶（SOD）和过氧化物酶（POD）活性，起到清除活性氧（ROS）、缓解Cd毒害和抑制丙二醛（MDA）积累的作用.最佳LED3A添加量下（50mg/L），黑麦草植株的总Cd累积量和转移系数较Cd单独处理时分别提高了74.39%和67.96%，Cd的去除率达到55.98%.研究表明添加LED3A可有效提高植物对Cd的修复效率.

Chelating surfactant assisted phytoremediation of Cd contaminated water by ryegrass

A novel chelating surfactant named N-lauroyl ethylenediamine triacetate (LED3A) assisted phytoremediation of Cd by ryegrass was studied under hydroponics conditions. Physiological responses of ryegrass, as well as the Cd removal efficiency, in Cd or LED3A alone and combination treatments were investigated. The results indicated that the biomass and photosynthetic pigments of ryegrass significantly declined under Cd stress. LED3A plus Cd addition would lead to slight increase of the inhibition mentioned above. The chlorophyll and carotenoid contents in ryegrass of CL100 treatment were decreased by 15.50% and 8.18% compared with CL0. No obvious changes of ryegrass biomass were observed with increasing LED3A concentration when LED3A was added alone. The relative electrical conductivity (REC) of ryegrass cells were increased by LED3A, which may increase the permeability of the cell membrane and hence promote Cd uptake by ryegrass. An appropriate quantity of LED3A could enhanced the activities of superoxide dismutase (SOD) and peroxide enzyme (POD) of ryegrass under Cd stress, suggesting a positive role of LED3A in scavenging reactive oxygen species (ROS), alleviating Cd toxicity and preventing malonyldialdehyde (MDA) accumulation. At optimal LED3A dosage (50mg/L), the total Cd accumulation amount and Cd translocation factor in ryegrass were increased by 74.39% and 67.96% compared with the Cd treatment alone. The optimal removal efficiency of Cd was reached 55.98%. The studies have shown that the addition of LED3A can effectively improve the phytoremediation efficiency of Cd in Cd contaminated water.