水源水库贫营养好氧反硝化菌群脱氮特性研究

为削减微污染水库中氮素的浓度，通过对西安市李家河水库沉积物进行定向富集驯化，筛选出以Pseudomonas菌为主、具有高效好氧反硝化特性的混合菌群-A1.摇床实验表明，贫营养好氧反硝化菌群A1在15h时硝酸盐氮去除率可达93.39%，硝酸盐氮平均去除速率为0.2073mg/（L·h）；总氮去除率为52.11%，总氮平均去除速率为0.1153mg/（L·h），无亚硝酸盐积累.氮平衡分析表明，约45%的初始氮被去除转化为气体产物.响应面法（RSM）结果表明，C/N比9.96，温度22.67℃，pH8.01，转速91r/min，溶解氧8.55mg/L是去除总氮（TN）的最优条件.

Study on the denitrifying characteristics of oligotrophicaerobic denitrifying bacteria flora from source water reservoir

In order to reduce the nitrogen concentration in micro-polluted reservoirs, a mixed high efficient aerobic denitrifying flora-A1, dominated by Pseudomonas, was directional enrichment and domestication from the sediments of Lijiahe Reservoir (Xi'an). Shaker experiments showed that the nitrate nitrogen removal ratio of lean nutrient aerobic denitrifying flora A1reached 93.39% at 15h, and the average nitrate removal rate was 0.2073mg/ (L·h). The total nitrogen removal ratio was 52.11% and the average removal rate of total nitrogen was 0.1153mg/(L·h). There was no nitrite accumulation detected. Nitrogen balance analysis showed that about 45% of the initial nitrogen was removed and converted into gas products. Response surface method (RSM) results showed when C/N ratio=9.96, temperature=22.67 ℃, pH=8.01, speed=91r/min, dissolved oxygen=8.55mg/L, the optimum condition of total nitrogen (TN) removal could be obtained.