微生物菌剂对砒砂岩土壤的改良作用

为了研究微生物菌剂改良砒砂岩土壤特性的效果，以植物促生细菌Bacillus halotolerans P75、Sinorhizobium meliloti D10、Bacillus megaterium H3和Bacillus subtilis HB01为原料制备4种液体单一菌剂和1种复合菌剂CB，分别按5%（体积质量比）接种到砒砂岩土壤中，种植紫花苜蓿和柠条，进行盆栽试验.通过分析植物生物量、土壤pH值、有机质、氮磷钾含量、酶活性和水稳性团聚体等指标来评价微生物菌剂对砒砂岩土壤的改良效果.结果表明，与不接菌的对照组相比，接菌处理能使紫花苜蓿显著增产18.6%~45.6%、柠条显著增产24.1%~46.7%，降低土壤pH值，促使土壤中的有机质、铵态氮、硝酸盐氮、速效磷、速效钾含量不同程度的增加，提高了土壤团聚体的水稳性，且接种复合菌对砒砂岩土壤的改善效果要优于单一菌株的效果.接种供试菌株能够改善砒砂岩的土壤质量，同时促进植物生长，其中复合菌对砒砂岩土壤的改良效果更为突出.

Effect of microbial inoculants on improvement of Pisha sandstone soil

In order to study the effect of microbial inoculants on improving the soil properties of Pisha sandstone soil, four single liquid microbial agents and one compound microbial agent CB were prepared from the plant growth-promoting bacteria Bacillus halotolerans P75, Sinorhizobium meliloti D10, Bacillus megaterium H3and Bacillus subtilis HB01, which were inoculated into Pisha sandstone soil at a volume-mass ratio of 5% respectively. The greenhouse pot experiments of planting Alfalfa (Medicogo sativa L.) and Caragana (Caragana korshinskii kom.) were conducted to evaluate the effects of the microbial agents on the Pisha sandstone soil quality and the growth of Alfalfa and Caragana. The results showed that the inoculated treatments could significantly increase Alfalfa biomass by 18.6%~45.6% and Caragana biomass by 24.1%~46.7% compared with the non-inoculated control group. Some bacterial treatments also could reduce soil pH, increase the contents of organic matter, ammonium nitrogen, nitrate nitrogen, available phosphorus and available potassium in soil, and improve water stability of soil aggregates. The compound bacterial agent could be more efficiently used for plant growth and the quality improvement of Pisha sandstone soil.