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Mutagenic and recombinagenic activity of airborne particulates, PM10 and TSP, organic extracts in the Drosophila wing-spot test |
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| Authors: | Dihl Rafael Rodrigues da Silva Carla Grazielli Azevedo do Amaral Viviane Souza Reguly Maria Luiza de Andrade Heloísa Helena Rodrigues |
| Affiliation: | a Programa de Pós Graduação em Genética e Biologia Molecular (PPGBM), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil b Instituto de Química, Departamento de Química Orgânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil c Laboratório de Diagnóstico da Toxicidade Genética (TOXIGEN), Programa de Pós Graduação em Genética e Toxicologia Aplicada (PPGGTA), Universidade Luterana do Brasil (ULBRA), Avenida Farroupilha 8001, 92420280 Canoas, RS, Brazil |
| Abstract: | The genotoxicity associated with air pollution in the city of Canoas, Rio Grande do Sul (Brazil), was assessed in November (spring) and January (summer). We applied the somatic mutation and recombination test (SMART) in Drosophila melanogaster in its standard version with normal bioactivation (ST) and in its variant with increased cytochrome P450-dependent biotransformation capacity (HB). The data indicated the genotoxicity of TSP and PM10 collected in November, in both ST and HB crosses. The genotoxic activity of the PM10 material in the spring sample was exclusively associated with the induction of mitotic recombination, whereas the TSP genetic toxicity was due to both recombinational as well as point and/or chromosomal mutation events. Considering PM10 collected in January, a positive response--100% (17.10 m3/ml) concentration--was observed in the HB cross, which was not detected in the ST cross. |
| Keywords: | SMART Genotoxicity Airborne particulates Drosophila melanogaster Mitotic recombination |
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