Differential response of multiple zebrafish hepatic F-box protein genes to17α-ethinylestradiol treatment

Differential response of multiple zebrafish hepatic F-box protein genes to 17alpha-ethinylestradiol treatment

Estrogens are accumulating in environment and their e ects on a variety of reproductive processes and tumorigenesis were reportedby previous study, but the mechanism of estrogen promoting neoplasia was still not clear. F-box protein (FBP) is the component of E3ubiquitin ligase which takes part in a variety of key biological processes. In this study, using mature male zebrafish, which are moresensitive to estrogen treatment, we examined influence of 17 -ethinylestradiol (EE2) exposure on the expression of a series of hepaticFBP genes, which take part in a variety of biological processes, including tumorigenesis. The influence of EE2 on the expression ofhepatic mRNA concentrations of FBP genes were quantified based on the expression of the optimal internal control gene in malezebrafish after 7-day exposure to EE2, from a low-dose concentration (1 ng/L) to environmentally relevant concentrations (10, 100ng/L). Our results showed that EE2 exposure reduced the expression of fbxl14a, fbxl14b, fbxo25 and -TRCP2b, but enchanced theexpression of skp2. While the alterations in fbxl2, fbxw7, fbxo9, -TRCP2a, fbxl18 and fbxo45 mRNA levels were not observed afterEE2 exposure. Thus, our results showed that the expression of hepatic FBP genes exhibited di erentially in male zebrafish exposedEE2. The changes of the expression level of FBP genes induced by EE2 may be an important clue to elucidate the correlations ofestrogen and hepatic tumors.