| 一种未见报道的厌氧氨氧化菌分子生物学鉴定 |
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| 引用本文： | 任宏洋,张代钧,丛丽影,蔡庆.一种未见报道的厌氧氨氧化菌分子生物学鉴定[J].环境工程学报,2008,2(3):314-318. |
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| 作者姓名： | 任宏洋 张代钧 丛丽影 蔡庆 |
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| 作者单位： | 1. 重庆大学环境科学系,重庆,400030
2. 重庆大学环境科学系,重庆,400030;重庆大学西南资源开发及环境灾害控制工程教育部重点实验室,重庆,400030 |
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| 基金项目： | 国家自然科学基金
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重庆大学校科研和教改项目 |
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| 摘 要： | 以(NH4):SO4和NaNO2作为基质,富集厌氧氨氧化污泥.提取厌氧氨氧化污泥中细菌总DNA,纯化后使用特异性引物对厌氧氨氧化菌16S rDNA进行PCR扩增.扩增产物连接到pMD19-T载体,将载体转化到感受态细胞大肠杆菌JM109中,并对其16S rDNA基因进行测序.将测序结果进行系统发育树分析,发现富集得到的厌氧氨氧化菌与Candidatus Anammoxoglobus propionicus进化关系比较接近,是一种尚未见报道的厌氧氨氧化菌.
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| 关 键 词： | 厌氧氨氧化菌 16S rDNA 鉴定 |
| 文章编号： | 1673-9108(2008)03-0314-05 |
| 修稿时间： | 1/9/2008 12:00:00 AM |
Identification of an unknown ANAMMOX bacterium by molecular biology |
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| Ren Hongyang,Zhang Daijun,Cong Liying and Cai Qing.Identification of an unknown ANAMMOX bacterium by molecular biology[J].Techniques and Equipment for Environmental Pollution Control,2008,2(3):314-318. |
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| Authors: | Ren Hongyang Zhang Daijun Cong Liying and Cai Qing |
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| Institution: | 1.Department of Environmental Science, Chongqing University, Chongqing 400030,1.Department of Environmental Science, Chongqing University, Chongqing 400030;2Key Laboratory of Southwest-China Resources Exploitation & Environmental Disaster Control Engineering, Ministry of Education, Chongqing University,Chongqing 400030,1.Department of Environmental Science, Chongqing University, Chongqing 400030 and 1.Department of Environmental Science, Chongqing University, Chongqing 400030 |
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| Abstract: | Anaerobic ammonium oxidation (ANAMMOX ) sludge was enriched with (NH4)2SO4 and NaNO2. Crude DNA of the total bacteria in cultivated anaerobic ammonium-oxidizing sludge was extracted and purified. Then partial 16SrDNA sequence of ANAMMOX bacterium was amplified by polymerase chain reaction(PCR) with a pair of specific primers. The PCR product was cloned into the vector pMD19-T and was transfected into competent cells E.coil JM109, then 16S rDNA sequence was determined. Phylogenetic analysis indicates that the cultivated ANAMMOX bacterium closes to Candidatus Anammoxoglobus propionicus, and has never been found. |
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