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A rapid and quantitative mass spectrometry method for determining the concentration of acylcarnitines and aminoacids in amniotic fluid |
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| Authors: | Laura Braida Sergio Crovella Michele Boniotto Ariella Luchesi Umberto de Vonderweid Bruno Casetta Antonio Amoroso |
| Institution: | 1. Servizio di Genetica Medica, IRCCS Burlo Garofolo, Via dell'Istria 5/1, 34137 Trieste, Italy
Dipartmento di Scienze della Riproduzione e dello Sviluppo, Università di Trieste, via dell'Istria 65/1, 34137 Trieste, Italy;2. Servizio di Genetica Medica, IRCCS Burlo Garofolo, Via dell'Istria 5/1, 34137 Trieste, Italy;3. Unità Operativa di Neonatologia, IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34137 Trieste, Italy Dipartmento di Scienze della Riproduzione e dello Sviluppo, Università di Trieste, via dell'Istria 65/1, 34137 Trieste, Italy;4. Applied Biosystems, Via Tiepolo 18, 20052 Monza (MI), Italy |
| Abstract: | We describe a quantitative, rapid, sensitive and reproducible tandem mass spectrometry (MSMS) method for the one-step detection of aminoacid (AAs) and acylcarnitine (ACs) concentrations in amniotic fluid. This technology is quicker and more sensitive than other methods used to date since it is possible to determine very low AA and AC concentrations in samples simultaneously in a single run. The high degree of automation allows a large number of pregnancies to be screened for metabolic defects in a very short time. Copyright © 2001 John Wiley & Sons, Ltd. |
| Keywords: | amniotic fluid aminoacids acylcarnitines mass spectrometry |