| Elevated 8-oxo-7,8-dihydro-2′-deoxyguanosine in genome of T24 bladder cancer cells induced by halobenzoquinones |
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| 作者姓名： | Tian Xu Junfa Yin Shaokun Chen Dapeng Zhang Hailin Wang |
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| 摘 要： | Halobenzoquinones(HBQs) are an emerging class of halogenated disinfection byproducts(DBPs) in drinking water, which raised public concerns due to potential carcinogenic effects to human bladder. Our previous work demonstrated that HBQs and hydrogen peroxide(H_2O_2)together generated oxidative DNA damage via a metal-independent and intercalationenhanced oxidation mechanism in vitro. This study further investigated the efficiency of various HBQs to induce oxidative DNA damage in T24 bladder cancer cells. Compared with T24 cells without treatment(3.1 lesions per 10~6 d G), the level of 8-oxo-7,8-dihydro-2′-deoxyguanosine(8-oxod G) significantly increased by 1.4, 3.2, 8.8, and 9.2 times after treatment with tetrabromo-1,4-benzoquinone(TBBQ), terachloro-1,4-benzoquinone(TCBQ),2,6-dichloro-1,4-benzoquinone(2,6-DCBQ) and 2,5-dichloro-1,4-benzoquinone(2,5-DCBQ) for24 hr, respectively. Interestingly, we found that the oxidative potency of HBQs in T24 cells(2,5-DCBQ ≈ 2,6-DCBQ TCBQ TBBQ) is inconsistent with that of in vitro ds DNA oxidation(TCBQ TBBQ 2,5-DCBQ 2,6-DCBQ), suggesting HBQs induce oxidative lesions in cellular genomic DNA probably involved with a complex mechanism.
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| 收稿时间： | 2017/1/23 0:00:00 |
| 修稿时间： | 2017/3/6 0:00:00 |
Elevated 8-oxo-7,8-dihydro-2'-deoxyguanosine in genome of T24 bladder cancer cells induced by halobenzoquinones |
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| Tian Xu,Junfa Yin,Shaokun Chen,Dapeng Zhang,Hailin Wang.Elevated 8-oxo-7,8-dihydro-2'-deoxyguanosine in genome of T24 bladder cancer cells induced by halobenzoquinones[J].Journal of Environmental Sciences,2018,30(1):133-139. |
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| Authors: | Tian Xu Junfa Yin Shaokun Chen Dapeng Zhang and Hailin Wang |
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| Institution: | 1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2.University of Chinese Academy of Sciences, Beijing 100049, China,1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2.University of Chinese Academy of Sciences, Beijing 100049, China,1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China and 1.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2.University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | |
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| Keywords: | Halobenzoquinones Reactive oxygen species Oxidative DNA damage 8-Oxo-7 8-dihydro-2- deoxyguanosine (8-oxodG) |
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