Coupled oxidation of aromatic hydrocarbons by horseradish peroxidase and hydrogen peroxide

The oxidation capability of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) coupled oxidation of aromatic hydrocarbons (o-xylene-d10 and naphthalene-d8) was investigated. Batch experiments were conducted using horseradish peroxidase prepared in potassium phosphate buffer in the presence of H2O2. The oxidation of aromatic hydrocarbon was tested as a function of HRP at a fixed concentration of H2O2, and as a function of the concentration of H2O2 at a constant HRP activity (4000 units/ml). The mass removal of o-xylene-d10 and naphthalene-ds increased with increasing HRP enzymatic activity, and up to 54% and 51% of mass removal were observed for o-xylene-d10 and naphthalene-d8, respectively. Increasing the concentration of H2O2 resulted in increased mass removal of aromatic hydrocarbons.