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Cupriavidus metallidurans SHE好氧还原Se(Ⅳ)合成硒纳米颗粒的特性研究
引用本文:沈娥,刘紫嫣,周豪,李端行,张旭旺,王经伟,沈文丽,曲媛媛,周集体.Cupriavidus metallidurans SHE好氧还原Se(Ⅳ)合成硒纳米颗粒的特性研究[J].环境科学学报,2015,35(10):3065-3070.
作者姓名:沈娥  刘紫嫣  周豪  李端行  张旭旺  王经伟  沈文丽  曲媛媛  周集体
作者单位:工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学盘锦校区食品与环境学院, 盘锦 124000,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024,工业生态与环境工程教育部重点实验室, 大连理工大学环境学院, 大连 116024
基金项目:国家自然科学基金(No.21176040);新世纪优秀人才支持计划(No.NCET-13-0077);中央高校基本科研业务费专项资金(No.DUT14YQ107)
摘    要:利用贪铜杆菌(Cupriavidu smetallidurans)SHE在好氧条件下还原Se(Ⅳ)生成硒纳米颗粒,考察不同条件对还原过程的影响,并对还原产物进行表征.结果显示,菌株SHE还原Se(Ⅳ)的最适条件为pH=8、温度30℃、底物浓度1.0 mmol·L-1,在此条件下Se(Ⅳ)的还原率最高,可达100%.通过紫外光谱扫描、微观形貌分析、粒度分析及X射线衍射分析表明,合成的硒纳米颗粒为六方晶型,粒径为(130.2±27.0)nm.研究结果表明,菌株SHE可有效的还原Se(Ⅳ)生成硒纳米颗粒,为微生物合成纳米硒的潜在应用提供参考.

关 键 词:贪铜杆菌  亚硒酸盐  硒纳米颗粒  微生物合成
收稿时间:2014/11/21 0:00:00
修稿时间:2014/12/21 0:00:00

Biosynthesis of selenium nanoparticles through aerobic selenite reduction by Cupriavidus metallidurans SHE
SHEN E,LIU Ziyan,ZHOU Hao,LI Duanxing,ZHANG Xuwang,WANG Jingwei,SHEN Wenli,QU Yuanyuan and ZHOU Jiti.Biosynthesis of selenium nanoparticles through aerobic selenite reduction by Cupriavidus metallidurans SHE[J].Acta Scientiae Circumstantiae,2015,35(10):3065-3070.
Authors:SHEN E  LIU Ziyan  ZHOU Hao  LI Duanxing  ZHANG Xuwang  WANG Jingwei  SHEN Wenli  QU Yuanyuan and ZHOU Jiti
Institution:Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Food and Environmental Science and Technology, Dalian University of Technology, Panjin 124000,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024 and Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024
Abstract:
Keywords:Cupriavidus metallidurans  selenite  selenium nanoparticles  microbial synthesis
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