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钝顶螺旋藻对Cd胁迫的生理反应
引用本文:王红叶,杨芳,陈思嘉,白燕,郑文杰. 钝顶螺旋藻对Cd胁迫的生理反应[J]. 海洋环境科学, 2007, 26(2): 151-153
作者姓名:王红叶  杨芳  陈思嘉  白燕  郑文杰
作者单位:1. 暨南大学,化学系,广东,广州,510632
2. 暨南大学,化学系,广东,广州,510632;暨南大学,水生生物研究所,广东,广州,510632
基金项目:广东省自然科学基金 , 教育部科学技术研究项目 , 广东省广州市科技攻关项目
摘    要:研究了钝顶螺旋藻在Cd胁迫下的生理指标的变化.结果表明,在CdCl2浓度为0~24 mg/L范围的Cd胁迫下,随着CdCl2浓度的增加,可溶性蛋白的含量明显低于对照组,抗氧化系统也受到了不同程度的影响.SOD(超氧化物歧化酶)和POD(过氧化物酶)活力呈先增后降趋势,其中POD活力增加的程度较大,最高可达对照的360%,最低也为对照组的262%.在0~24 mg/L浓度范围内,APX(抗坏血酸氧化酶)活力均低于对照组,但随CdCl2浓度的增加呈递增状态.CAT(过氧化氢酶)均高于对照组,整体呈增加的趋势.GSH-PX(谷胱甘肽过氧化物酶)活力均低于对照,最低降至对照组的25%.MDA(丙二醛)作为膜脂质过氧化的产物,其累积是Cd胁迫的突出的生理变化之一,MDA的含量与CdCl2的浓度呈正相关.

关 键 词:钝顶螺旋藻  抗氧化  SOD  POD  MDA  钝顶螺旋藻  胁迫  生理反应  Spirulina platensis  相关  生理变化  膜脂质过氧化  丙二醛  胱甘肽过氧化物酶  过氧化氢酶  状态  递增  抗坏血酸氧化酶  浓度范围  趋势  超氧化物歧化酶  影响  程度  氧化系统  对照组
文章编号:1007-6336(2007)02-0151-03
收稿时间:2005-09-21
修稿时间:2005-12-12

Physiological responses to Cd2+ stress in Spirulina platensis
WANG Hong-ye,YANG Fang,CHEN Si-jia,BAI Yan,ZHENG Wen-jie. Physiological responses to Cd2+ stress in Spirulina platensis[J]. Marine Environmental Science, 2007, 26(2): 151-153
Authors:WANG Hong-ye  YANG Fang  CHEN Si-jia  BAI Yan  ZHENG Wen-jie
Affiliation:1. Department of Chemistry, Jinan University, Guangzhou 510632, China; 2. Insititute of Hydrobiology, Jinan University; Guangzhou 510632, China
Abstract:Under Cd2 stress,some physiological properties were changed in Spirulina platensis.Results showed that,between 0 to 24 mg/L CdCl2,soluble protein contents were all below the control,and the antioxidant systems were affected,too.Superoxide dismutase(SOD) and peroxidase(POD) were both induced in high level,moreover,the activities increased at first,and then decreased.The activity of ascorbate peroxidase(APX) increased when Cd2 concentration increased,however,all of APX were below the control.Meanwhile,the activities of catalase(CAT) were all above the control,and increased along with Cd2 concentration increasing.The activities of glutathione peroxidase(GSH-PX) were all below the control,and the least decreased to 25%.The remarkable enhancement of malonyl dialdehyde(MDA) appeared to be an obvious physiological response due to Cd2 treatment.There was a close positive correlation between MDA content and Cd2 concentration.
Keywords:Spirulina platensis  antioxidation  SOD  POD  MDA
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