| 利用脂肪酶提高鱼油中多不饱和脂肪酸(PUFAs)甘油酯 |
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| 引用本文： | 郑毅,郑楠,卓进锋,马霖霖. 利用脂肪酶提高鱼油中多不饱和脂肪酸(PUFAs)甘油酯[J]. 应用与环境生物学报, 2005, 11(5): 571-574 |
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| 作者姓名： | 郑毅 郑楠 卓进锋 马霖霖 |
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| 作者单位： | 1. 福建师范大学生物工程学院,福州,350007;福建农林大学生物技术中心,福州,350002
2. 福建师范大学生物工程学院,福州,350007 |
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| 基金项目： | 福建省自然科学基金资助项目(B0210005)~~ |
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| 摘 要： | 利用脂肪酶选择性的水解作用研究提高鱼油中多不饱和脂肪酸(PUFAs)甘油酯的含量.通过对5种不同来源脂肪酶的筛选,以来源于米曲霉脂肪酶为最佳酶种;同时研究该酶水解鱼油的最佳工艺条件:反应温度、加酶量、反应转速、添加剂影响;最后确定最佳的富集PUFAs甘油酯时间.在此工艺条件下,鱼油中EPA由3.0%提高到9.0%,DHA由4.3%提高到16.5%,EPA DHA由7.3%提高到25.5%.图3表1参8
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| 关 键 词： | 脂肪酶 多不饱和脂肪酸 水解 浓缩 鱼油 |
| 收稿时间： | 2004-09-10 |
| 修稿时间： | 2004-11-01 |
CONCENTRATION OF PUFAs IN GLYCERIDE BY HYDROLYSIS OF FISH OIL WITH LIPASE |
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| ZHENG Yi,ZHENG Nan,ZHUO Jinfeng,MA Lingling. CONCENTRATION OF PUFAs IN GLYCERIDE BY HYDROLYSIS OF FISH OIL WITH LIPASE[J]. Chinese Journal of Applied and Environmental Biology, 2005, 11(5): 571-574 |
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| Authors: | ZHENG Yi ZHENG Nan ZHUO Jinfeng MA Lingling |
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| Affiliation: | 1. College of Bio-engineering, Fujian Normal University, Fuzhou 350007, China;2. Centre of Biotechnology Research, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | Increasing PUFAs (polyunsaturated fatty acids) in glyceride of fish oil by selective hydrolysis with lipase was studied. Through selection of 5 lipases from different sources, the lipase from Aspergillus oryzae showed the best quality. The conditions for hydrolysis of fish oil with this lipase were optimized, including temperature, rotate speed, ratio of oil to water, amount of lipase and additives. Finally, the optimum time for enriching PUFAs in glyceride was confirmed. The results showed under the hydrolysis conditions the content of EPA was increased from 3.0 % to 9.0 %, DHA from 4.3 % to 16.5 %, and DHA EPA from 7.3 % to 25.5 %. Fig 3, Tab 1, Ref 8 |
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| Keywords: | lipase PUFAs hydrolysis concentration fish oil |
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