大连星海浴场中大肠杆菌耐药性分析

研究了大连星海浴场中大肠杆菌的耐药性,对从海水中分离出的36株疑似大肠杆菌进行生理生化鉴定和分子学鉴定发现8株为大肠杆菌,并采用K-B纸片法测定了其对12种常见抗生素的耐药性。药敏实验结果显示,所有大肠杆菌菌株均表现出耐药性,并对12种所选抗生素中的10种抗生素的耐药率超过了50%,其中,对利福平的耐药率为100%;而对复方新诺明,氯霉素的耐药率分别为37.5%和25%。通过分析大肠杆菌耐药谱发现,100%的大肠杆菌菌株表现出了多重耐药性,其中,87.5%的大肠杆菌对3种及其以上的抗生素表现出抗性。上述结果表明大连星海浴场中大肠杆菌的多重耐药状况严重。

Antibiotic resistance of Escherichia coli in Dalian Xinghai Bathing Beach

We study the antibiotic resistance of Escherichia coli in Dalian Xinghai Bathing Beach.36 strains were screened from seawater using membrane filter and used for further identification.Biochemical method and PCR method confirmed 8 of 36 strains were Escherichia coli.Antibiotic resistance of E.coli to 12 common-used antibiotics were measured using K-B paper test.All tested E.coli showed antibiotic resistance and resistant rates is over 50% to 10 of 12 antibiotics.The resistant rates of E.coli to rifampicin,chloramphenicol and Compound sulfamethoxazole is 100%,25%and 37.5%,respectively.All isolated E.coli were identified as multiple-resistant strains,87.5% of E.coli occurred resistant to more than 3 antibiotics.These results suggest the multiple-resistance of E.coli is severe in Dalian Xinghai Bathing Beach.