固相萃取-高效液相色谱法测地表水中11种酚类化合物

建立了固相萃取-高效液相色谱法同时测定地表水中11种酚类化合物的方法。水样经过全自动固相萃取仪富集,以HLB柱为萃取柱,乙腈(含1%乙酸)为洗脱剂,用高效液相色谱仪分析定量。该方法在0.5~5.0 mg/L范围内线性良好,相关系数为0.999 6~0.999 9,11种酚类化合物的纯水加标回收率为82.0%~111%,地表水加标回收率为98.5%~116%,精密度为3.58%~4.67%,检出限为1×10-4~5×10-4mg/L,该方法简单实用、准确可靠,可用于地表水中酚类化合物的同时测定。

Determination of Eleven Phenolic Compounds in Surface Water by Solid-Phase Extraction and High Performance Liquid Chromatography

A high performance liquid chromatography (HPLC) method was established for the determination of eleven phenolic compounds in surface water. The sample was enriched with solid-phase extraction column, extracted with acetonitrile (1% acetic acid) and then analyzed by HPLC. Under the optimal conditions, good linearities were obtained in the range of 1×10^-4 -5×10^-4 mg/L with correlation coefficients of 0.999 6-0.999 9.The recoveries of eleven phenolic compounds of pure water and surface water were 82.0%-111% and 98.5%-116%.The relative standard deviations ranged from 3.58%to 4.67%.The limits were 1×10^-4-5×10^-4 mg/L. The method was easy and reliable. It can be used to determinate the phenolic compounds in surface water at the same time.