基于固定化AChE的流动注射型酶传感器研究

以固定化鲅鱼脑组织乙酰胆碱酯酶acetylcholinesterase,AChE(EC 3.1.1.7)]为识别元件,以pH电极为换能器,构建流动注射型AChE酶传感器.该传感器在以磷酸盐缓冲液为载液的条件下具有良好的重现性(RSD=1.427%,n=10)和敏感性,可实现对有机磷化合物的在线监测;对甲基对硫磷具有线性响应的浓度范围为4.29×10-10^-4.29×10^-8mol·L^-1,最低检测限为1.3×10^-

Flow Injection Biosensor Based on the Immobilized AChE

A biosensor based on the flow injection system was constructed with the immobilized AChE from Scomberomorus niphonius (Curier) as identification element and a pH electrode as transducer. When phosphate buffer was used as carrier liquid, a good reproducibility (RSD = 1.427% , n=10) of the biosensor response was obtained after the substrate was injected repetitively. After an incubation time of 20 min, the calibration graph to methyl-parathion is linear (r = 0.9986) when its concentration ranges from 4.29 x 10(-10) mol x L(-1) to 4.29 x 10(-8) mol x L(-1) , and the detection limit is 1.3 x 10(-10) mol x L(-1). However, the sensitivity of this biosensor to methyl-parathion when using clean seawater as carrier liquid is not as good as that using phosphate buffer. But after preoxidation of methyl-parathion with NaClO as oxidant, the detection limit of the biosensor to methyl-parathion in seawater can be improve to 2.16 x 10(-7) mol x L(-1).