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| 一株苯酚降解菌的分离和鉴定 | |
| 引用本文： | 徐玉泉,张维,陈明,林敏,李浚明,方宣钧. 一株苯酚降解菌的分离和鉴定[J]. 环境科学学报, 2000, 20(4): 450-455 |
| 作者姓名： | 徐玉泉 张维 陈明 林敏 李浚明 方宣钧 |
| 作者单位： | 1. 中国农科院生物技术研究所,北京,100086;中国农业大学生物学院,北京,100094;中国农科院原子能利用研究所,北京,100094 2. 中国农科院原子能利用研究所,北京,100094 3. 中国农业大学生物学院,北京,100094 4. 中国农科院生物技术研究所,北京,100086 |
| 基金项目： | 国家高科技 (86 3)计划资助 |
| 摘 要： | :从工业废水中分离到一株高效降解苯酚的菌株PHEA 2 ,它能够在以苯酚为单一碳源和能源的无机培养基上生长 ,其最高苯酚降解率为 394nmol/ (mg·min) .经Biolog细菌自动鉴定仪鉴定 ,该菌株为醋酸钙不动杆菌 (Acinetobactercalcoaceti cus) .采用PCR扩增获得的该菌 16SrDNA片段 ,核苷酸序列分析结果表明 ,该菌株的 16SrDNA的核苷酸序列与醋酸钙不动杆菌同源性为 98% .在细菌系统发育分类学上 ,PHEA 2归属变形细菌γ亚类、不动杆菌属、醋酸钙不动杆菌 . |
| 关 键 词： | 苯酚 生物降解 菌株 PHEA－2 分离 鉴定 废水处理 |
| 收稿时间： | 1999-07-19 |
| 修稿时间： | 1999-07-19 |
Isolation and identification of a phenol degrading bacterial strain |
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| XU Yuquan,ZHANG Wei,CHEN Ming,LIN Min,LI Junming and FANG Xuanjun. Isolation and identification of a phenol degrading bacterial strain[J]. Acta Scientiae Circumstantiae, 2000, 20(4): 450-455 | |
| Authors: | XU Yuquan ZHANG Wei CHEN Ming LIN Min LI Junming FANG Xuanjun |
| Affiliation: | 1. Institute of Biotechnology Reseach Center, CAAS, Beijing 100081; 2. College of Biology, CAU, Beijing 100094; 3. Institute for Application of Atomic Energy, CAAS, Beijing 100094,Institute for Application of Atomic Energy, CAAS, Beijing 100094,Institute for Application of Atomic Energy, CAAS, Beijing 100094,Institute for Application of Atomic Energy, CAAS, Beijing 100094,College of Biology, CAU, Beijing 100094 and Institute of Biotechnology Reseach Center, CAAS, Beijing 100081 |
| Abstract: | A strain that can grow with phenol as the sole source of carbon and energy, PHEA|2, was isolated from the wastewater discharged by an oil refinery. The maximum rate of phenol degradation of PHEA|2 is 394 nanomoles per minute per milligram of protein. Biolog Microstation Identification System was used to identify the PHEA|2 as Acinetobacter Calcoaceticus. The sequence analysis of a 1500 bp 16?S rDNA fragment amplified from total DNA of PHEA|2 strain by PCR showed that PHEA|shares 98% 16?S 4DNA sequence homology with typical %A.Calcoaceticus% strain. In phylogenetic framework of bacterial classification, PHEA|2 belongs to proteobacteria; gamma subdivision: ?inetobacter, A. Calcoaceticus.% |
| Keywords: | phenol biodegradation 16?S rDNA ?inetobacter calcoaceticus% |
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