利玛原甲藻中大田软海绵酸和鳍藻毒素-1提取与纯化

批量培养产生腹泻性贝类毒素的利玛原甲藻，甲醇提取超声破碎后的藻细胞；用大孔吸附树脂HP20富集其胞外毒素并进行毒素的初步纯化。经制备型高效液相色谱法分离，分别得到毫克级的大田软海绵酸和鳍藻毒素-1固体。经核磁共振氢谱和高分辨质谱确认结构，定量核磁确定纯度，得到的大田软海绵酸和鳍藻毒素-1纯度分别为99.39%和99.26%，满足标准物质制备要求。

Extraction and purification of okadaic acid (OA) and dinophysis toxin-1 (DTX1) in the dinoflagellate Protocentrum Lima

Marine dinoflagellate Prorocentrum lima producing diarrhetic shellfish poison were mass cultured and crushed by ultrasonic, followed by extracting with methanol for okadaic acid (OA) and dinophysis toxin-1(DTX1). OA and DTX1 with high purity were obtained by a preparative high performance liquid chromatographic (HPLC) method after enrichment and purification with macroporous adsorption resin HP20, which was used for the enrichment of extracellular toxin secreted in the sea water. The two compounds were identified by nuclear magnetic resonance hydrogen spectrum (1H-NMR) and high-resolution mass spectrometry (HRMS). The purity of OA and DTX1 was identified by quantitative nuclear magnetic to be 99.39% and 99.26% respectively, meeting the requirements for preparation of standard material.