Combined use of earthworm (Alma millsoni) and bacterium (Bacillus sp.) improved the bioremediation of spent engine oil contaminated soil |
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| Authors: | Akindele Oluwatosin Adeyi Lotanna Micah Nneji Esther Olubisi Adeyi Oluwatobi T. Somade Babatunde Kazeem Agbaogun |
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| Affiliation: | 1. Animal Physiology Unit, Department of Zoology, University of Ibadan, Ibadan, Nigeria;2. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, People’s Republic of China;3. Department of Biochemistry, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria;4. Department of Analytical and Ecological Chemistry, University of Trier, Trier, Germany |
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| Abstract: | This study was carried out to assess the effectiveness of the combined use of earthworm (Alma millsoni) and bacterium (Bacillus sp.) in the bioremediation of spent engine oil (SEO) contaminated soils. A. millisoni were collected from the Botanical Garden of University of Ibadan, Nigeria. The stock culture of hydrocarbonoclastic Bacillus sp. was used for the bioremediation study. A set-up of eight pots containing 1000?g soil sample and 20?g of cow dung were mixed with 100, 75, 50 and 0?mL SEO respectively. Each of the set up was subjected to bioremediation agents; A. millisoni alone, Bacillus sp alone, A. millisoni and Bacillus sp, no treatment (control) in duplicate. Treatment of 100?ml SEO contaminated soil with combined A. millisoni and Bacillus sp resulted in significantly (P? bacterium > vermi-remediating agents. Earthworms exposed to 100?mLSEO-contaminated soil had higher CAT, SOD, and GPx activities compared to the control. Findings indicated that A. millisoni with Bacillus sp. can synergistically improve bioremediation of SEO contaminated soils. |
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| Keywords: | Bioremediation Bacillus sp Alma millsoni spent engine oil oxidative stress |
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