| 快速测定水中大肠菌群的方法研究 |
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| 引用本文： | 王萍,梁霞,张豫川. 快速测定水中大肠菌群的方法研究[J]. 环境科学与技术, 2009, 32(4) |
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| 作者姓名： | 王萍 梁霞 张豫川 |
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| 作者单位： | 中国工程物理研究院核物理与化学研究所,四川,绵阳,621900;中国工程物理研究院核物理与化学研究所,四川,绵阳,621900;中国工程物理研究院核物理与化学研究所,四川,绵阳,621900 |
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| 摘 要： | 水质常规监测中,大肠菌群的检测采用的是传统的多管发酵法,由于传统方法费时费力,在水样多及时间紧的情况下,越来越不能满足实际监测的需要,本研究工作旨在建立一种快速检测的新方法,即在初发酵管中加入一定浓度的革兰氏阳性菌抑菌剂和显色剂,直接依据产酸产气及溶液颜色的变化,推算出水样中大肠菌群的含量。通过大量对比实验,最终完成了革兰氏阳性菌抑菌剂种类、浓度和显色剂浓度的确定工作,并用所购菌种进行了对比实验,结果证明,该方法准确性较好,与传统的多管发酵法进行比对,两种方法所测结果经统计学检验无显著性差异。
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| 关 键 词： | 大肠菌群 革兰氏阳性菌抑菌剂 显色剂TTC 平板培养法 |
Rapid Detection Method of the Coliform Bacteria in Water |
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| WANG Ping,LIANG Xia,ZHANG Yu-chuan. Rapid Detection Method of the Coliform Bacteria in Water[J]. Environmental Science and Technology, 2009, 32(4) |
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| Authors: | WANG Ping LIANG Xia ZHANG Yu-chuan |
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| Affiliation: | Institute of Nuclear Physics and Chemistry;China Academy of Engineering Physics;Mianyang 621900;China |
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| Abstract: | In the regular monitoring of water quality, poly-tube-zymolysis method cannot meet the demands of the short time and many specimens because takes much time.The new method of rapid detection was introduced, the concentration and species of GPBB(Gram-positive bacteria bacteriostat) were studied as well as concentration and color developing effect of chromogenic agent.The optimized concentration of GPBB and TTC(chromogenic agent) were obtained by the nutrient agar contrast experiment.The newly developed detect... |
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| Keywords: | coliform bacteria Gram-positive bacteria bacteriostat TTC plate culture |
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