联苯胺对锦鲤肝脏酶活性的影响

以锦鲤(Cyprinus carprio)为试验生物,经不同浓度(6.0,3.0,2.0,1.2,0.6mg/L)联苯胺暴露14d,研究其对肝脏谷胱甘肽过氧化物酶(GSH-Px),过氧化氢酶(CAT),超氧化物歧化酶(SOD)和谷胱甘肽硫转移酶(GST)以及谷丙转氨酶(GPT)活性的影响.结果表明,锦鲤经联苯胺暴露,肝脏GSH-Px活性几乎持续受抑制;暴露第4d出现CAT的诱导,但随着暴露的延续,在第7,14d出现酶活性抑制;对SOD的影响多表现为诱导作用,但在高浓度(6.0,3.0mg/L)暴露第14d则出现酶活性抑制;对GST和GPT的影响一般表现为在低浓度暴露下的酶诱导,高浓度暴露下酶活性受抑制.GSH-Px对联苯胺暴露最为敏感,有可能成为评价水环境联苯胺污染的生物标志物.

Effects of benzidine to the hepatic enzymes of brocaded carp (Cyprinus carprio)

Using brocaded carp (Cyprinus carprio) as test organism exposed to different concentrations of benzidine (6.0,3.0,2.0,1.2,0.6mg/L) for 14d, the influence on the activities of liver glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), glutathione s-transferase (GST), and glutamic-pyruvate transaminase (GPT) were studied. Brocaded carp after benzidine exposure even low concentration exposure, the liver GSH-Px activity almost all was inhibited continuously; after the exposure of fourth day, appeared the inducement of CAT, but with continuing of exposure, in seventh and fourteenth days, appeared inhibiliting of enzyme activity; the influence on SOD appeared generally inducing action, but at the fourteen day of high concentration exposure (6.0, 3.0mg/L) appeared inhibiting of enzyme activity; the influence on GST and GPT appeared inducing action under low concentration and inhibiting action under high concentration. GSH-Px was most sensitive to benzidine exposure, possible forming the biomarker of evaluating the benzidine pollution in aquative environment.