| 一种能原位检测类雌激素暴露的胶体金免疫检测试片 |
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| 引用本文： | 杨方星,徐盈.一种能原位检测类雌激素暴露的胶体金免疫检测试片[J].生态毒理学报,2007,2(2):220-224. |
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| 作者姓名： | 杨方星 徐盈 |
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| 作者单位： | 中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,武汉,430072 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 利用免疫层析和胶体金标记技术试制出一种免疫检测试片,该试片可快速定性检测鱼体内诱导的卵黄蛋白原.试验结果表明,该检测试片仅需采鱼血浆100μL,15min内便可获得检测结果.试片的检测限(LODVTG)为10μg·mL-1,野外现场应用表明测量准确度达92%.因此,该试片可用于野外水体类雌激素暴露与影响的原位评价.
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| 关 键 词： | 胶体金 卵黄蛋白原 类雌激素 检测试片 |
| 文章编号： | 1673-5897（2007）2-220-05 |
| 收稿时间： | 3/1/2007 12:00:00 AM |
| 修稿时间： | 2007-03-01 |
An Immunochromatographic Test Strip for in situ Identification of Vitellogenin Induction in Fish Exposed to Xenoestrogens |
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| YANG Fang-xing and XU Ying.An Immunochromatographic Test Strip for in situ Identification of Vitellogenin Induction in Fish Exposed to Xenoestrogens[J].Asian Journal of Ecotoxicology,2007,2(2):220-224. |
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| Authors: | YANG Fang-xing and XU Ying |
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| Institution: | State Key Laboratory of Freshwater Eeology and Biotechnology, Institute of Hydrobiology, Chinese Aeademy of Seienees, Wuhan 430072 |
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| Abstract: | An immunochromatographic test strip based on gold colloid was developed to quickly identify vitellogenin(VTG)induction in fish in field waters. The results indicated that the immunochromatographic test strip could perform the analysis with 100μL plasma in 15min. The limit of detection(LODVTG)of the immunochromatographic test strip was 10μg·mL-1 and the accuracy reached 92%. Therefore, it is proposed that the test strip can be a primary means for in situ assessing the effect of xenoestrogen exposure. |
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| Keywords: | gold colloid vitellogenin xenoestrogen test strip |
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