Antioxidant defense gene analysis in <Emphasis Type="Italic">Brassica oleracea</Emphasis> and <Emphasis Type="Italic">Trifolium repens</Emphasis> exposed to Cd and/or Pb |
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Authors: | F?Bernard S?Dumez F?Brulle S?Lemière A?Platel F?Nesslany D?Cuny A?Deram Email author" target="_blank">F?VandenbulckeEmail author |
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Institution: | 1.Université de Lille,Lille,France;2.Laboratoire de Génie Civil et géo-Environnement EA4515,Université Lille Nord de France-Lille 1,Villeneuve d’Ascq,France;3.Laboratoire des Sciences Végétales et Fongiques, Faculté des Sciences Pharmaceutiques et Biologiques,Université de Lille,Lille Cedex,France;4.Laboratoire de Toxicologie Génétique,Institut Pasteur de Lille,Lille,France;5.Faculté de Management de la Santé (ILIS),Université de Lille,Loos,France |
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Abstract: | This study focused on the expression analysis of antioxidant defense genes in Brassica oleracea and in Trifolium repens. Plants were exposed for 3, 10, and 56 days in microcosms to a field-collected suburban soil spiked by low concentrations of cadmium and/or lead. In both species, metal accumulations and expression levels of genes encoding proteins involved and/or related to antioxidant defense systems (glutathione transferases, peroxidases, catalases, metallothioneins) were quantified in leaves in order to better understand the detoxification processes involved following exposure to metals. It appeared that strongest gene expression variations in T. repens were observed when plants are exposed to Cd (metallothionein and ascorbate peroxidase upregulations) whereas strongest variations in B. oleracea were observed in case of Cd/Pb co-exposures (metallothionein, glutathione transferase, and peroxidase upregulations). Results also suggest that there is a benefit to use complementary species in order to better apprehend the biological effects in ecotoxicology. |
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