一株氯苯降解新菌株的分离鉴定及其降解特性研究

采集某净化氯苯废气的生物滴滤床填料表面的生物膜,分离纯化后得到1株能高效降解氯苯的菌株L2,基于菌株生理生化特征、16S rDNA序列系统学分析和Biolog鉴定,可确定该菌株为皮氏罗尔斯顿菌(Ralstonia pickettii),其为新发现的具有氯苯降解性能的菌株.该菌株最佳生长温度和pH分别为30 ℃和7,在最...

Isolation,Identification and Biodegradation Characteristics of a Novel Chlorobenzene-degrading Bacterial Strain

A bacterial strain L2 able to efficiently degrade chlorobenzene was isolated from biotrickling filer treating waste gas containing chlorobenzene.Based on physiological and biochemical characteristics,sequence analysis of 16S rDNA and Biolog identification system,the isolation strain was identified as Ralstonia pickettii which was possible a new species able to degrade chlorobenzene.The optimal conditions for growth of the strain were at 30 ℃ and pH 7.0.Under the optimal growth conditions,the removal efficiency up to 99% for 220 mg/L of chlorobenzene was obtained within 3 days.Yeast extract(YE)could remarkably facilitate the growth of L2 and chlorobenzene degradation.Adding YE of 10,20,50,100 and 150 mg/L,the specific growth rate of L2 would increase from 0.14 h-1 to 0.23 h-1,while average degradation rate of chlorobenzene by L2 was correspondingly increased from 3.03 mg/h to 4.40 mg/h.Based on preliminary determination of catechol dioxygenase activity,it was proposed that chlorobenzene was converted to pyrocatechol during the biodegradation,and ring cleavage of pyrocatechol was primarily via catechol 1,2-dioxygenase.