| 纳米富勒烯对土壤酶活性和微生物群落的影响 |
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| 引用本文： | 付宇童,高珊,王伟全,徐应明,孙约兵. 纳米富勒烯对土壤酶活性和微生物群落的影响[J]. 环境科学学报, 2020, 40(1): 242-250. DOI: 10.13671/j.hjkxxb.2019.0336 |
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| 作者姓名： | 付宇童 高珊 王伟全 徐应明 孙约兵 |
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| 作者单位： | 东北农业大学资源与环境学院,哈尔滨150030;农业农村部环境保护科研监测所,农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191,东北农业大学资源与环境学院,哈尔滨150030;农业农村部环境保护科研监测所,农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191,东北农业大学资源与环境学院,哈尔滨150030;农业农村部环境保护科研监测所,农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191,农业农村部环境保护科研监测所,农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191,农业农村部环境保护科研监测所,农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191 |
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| 基金项目： | 天津市自然科学基金重点项目（No.17JCZDJC34200）；中国农业科学院基本科研业务费专项院级统筹项目（No.Y2018PT12） |
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| 摘 要： | 采用室内暗培养试验,研究了纳米富勒烯对土壤呼吸强度、酶活性及微生物结构群落多样性的影响.结果表明,添加纳米富勒烯抑制了土壤呼吸强度(p0.05),较对照处理CO_2累积量降低了1.2%～11.3%.纳米富勒烯显著抑制了土壤脲酶活性(p0.05),与对照相比,降低了19.1%～33.7%,土壤脱氢酶活性整体上表现为随纳米富勒烯添加量增加先增加后降低的趋势.与之相反,添加不同剂量纳米富勒烯整体上提高了土壤碱性磷酸酶活性,但差异不明显(p0.05).利用磷脂脂肪酸(Phospholipid Fatty Acids, PLFAs)分析发现,添加纳米富勒烯(10～500 mg·kg~(-1))后土壤总PLFAs含量较对照降低了3.6%～27.3%,在低剂量(≤50 mg·kg~(-1))纳米富勒烯处理下革兰氏阳性细菌、革兰氏阴性细菌、真菌、细菌含量均有所提升,而当纳米富勒烯剂量达到500 mg·kg~(-1)时,土壤微生物数量在不同程度上受到抑制.土壤微生物生态学指数(Shannon、Simpson和Pielou指数)、聚类分析(Cluster analysis)、主成分分析(Principal component analysis)均显示纳米富勒烯对土壤中微生物群落多样性产生了负面影响.
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| 关 键 词： | 纳米富勒烯 土壤 呼吸强度 酶活性 磷脂脂肪酸 |
| 收稿时间： | 2019-06-17 |
| 修稿时间： | 2019-08-21 |
Effects of nano-fullerene on soil enzyme activities and microbial community |
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| FU Yutong,GAO Shan,WANG Weiquan,XU Yingming and SUN Yuebing. Effects of nano-fullerene on soil enzyme activities and microbial community[J]. Acta Scientiae Circumstantiae, 2020, 40(1): 242-250. DOI: 10.13671/j.hjkxxb.2019.0336 |
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| Authors: | FU Yutong GAO Shan WANG Weiquan XU Yingming SUN Yuebing |
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| Affiliation: | 1. College of Resources and Environment, Northeast Agricultural University, Harbin 150030;2. Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs(MARA)/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Agro-Environmental Protection Institute, MARA, Tianjin 300191,1. College of Resources and Environment, Northeast Agricultural University, Harbin 150030;2. Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs(MARA)/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Agro-Environmental Protection Institute, MARA, Tianjin 300191,1. College of Resources and Environment, Northeast Agricultural University, Harbin 150030;2. Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs(MARA)/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Agro-Environmental Protection Institute, MARA, Tianjin 300191,Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs(MARA)/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Agro-Environmental Protection Institute, MARA, Tianjin 300191 and Key Laboratory of Original Agro-Environmental Pollution Prevention and Control, Ministry of Agriculture and Rural Affairs(MARA)/Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety, Agro-Environmental Protection Institute, MARA, Tianjin 300191 |
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| Abstract: | |
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| Keywords: | nano-fullerene soil respiration intensity enzymes activity phospholipid fatty acids |
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