固定在活性炭聚砜中空纤维膜中的Pseudomonas putida菌对四氯苯酚的共代谢降解

以苯酚-四氯苯酚共代谢体系为对象,研究了中空纤维聚砜膜作为细菌固定化材料对该共代谢过程的强化作用.结果表明,该膜具有内、中、外3层的结构,Pseudomonas putida菌可被固定在膜的表面和中间层;固定化后,细菌对高浓度有毒底物的忍受限度增强,从而得以持续生长,并在29 h内将600　mg/L和120　mg/L的苯酚和四氯苯酚完全降解.在膜的制作过程中添加了一定量的活性炭后,发现膜对苯酚和四氯苯酚的吸附能力增强,同时结构上更疏松,对四氯苯酚的降解效率得到了提高.固定在活性碳中空纤维膜中的Pseudomonas putida可以将1 000　mg/L和200　mg/L的苯酚和四氯苯酚在51 h内完全降解,比不加活性炭的情况缩短了37 h,中空纤维膜可以连续多次使用,对相同浓度的四氯苯酚的降解速度保持稳定.

Enhancement of Biodegradation of 4-chlorophenol During Co-metabolic Process by Immobilized-Cells of Pseudomonas putida

Cometablic biodegradation of phenol and 4-chlorophenol was enhanced by cells immobilized in polysulfone hollow fiber membranes. Polysulfone hollow fiber membranes have three layers in structure. Pseudomonas putida can be immobilized in the fibers and cometabolically degrade 600 mg/L phenol and 120 mg/L 4-chlorophenol (4-cp) within 29 hours. With the incorporation of activated carbon, the fibers show higher adsorption capacity and looser structure. 1000 mg/L phenol and 200 mg/L 4-cp were biodegraded within 51 hours by cells immobilized in activated carbon integrated hollow fiber membranes, while it took about 88 hours without activated carbon present. The immobilized cells can be used for at least four times. Immobilized cells were protected by hollow fiber membranes and transformed phenol and 4-cp continuously.