外源一氧化氮对亚心形扁藻的生长效应及光谱研究

对海洋绿藻亚心形扁藻(Platymonas subcordiformis)分别进行了不同一氧化氮(NO)浓度和光照强度的培养实验,并进行了叶绿素a和类胡萝卜素含量测定,及室温吸收光谱和荧光光谱测定.结果表明,不同NO浓度、不同加入方式对亚心形扁藻生长有明显不同的促进或抑制作用,NO对亚心形扁藻生长的影响存在阈值.不同浓度NO在不同光照下对微藻生长的作用是一致的,外源NO可以弥补弱光照对藻生长的限制.NO对亚心形扁藻光合色素含量的影响和对藻密度的影响是一致的.NO对藻细胞中叶绿素复合蛋白组成没有显著影响,但对色素含量及其相对组成会产生一系列影响.NO能使藻细胞激发能的传递效率提高,从而提高光合作用速率,表现为细胞生长加快,藻细胞生物量增加.

Growth Effect of Exogenous Nitric Oxide on Platymonas subcordiformis and Spectrum Study

Experiments on the effects of nitric oxide(NO) on the growth of marine green algae Platymonas subcordiformis were conducted,under the condition of different NO concentrations and illumination intensity respectively. The chlorophyll-a(Chl-a) and carotenoid contents of algae were measured,and the absorption spectrum and fluorescence spectrum under the room temperature were also determined.The results are as follows: The growth of Platymonas subcordiformis was obviously promoted or inhibited when different concentrations of NO was added only once or twice a day during the cultivation.So there are NO threshold concentrations for algae growth.Under the different illumination,the influence of different NO concentrations on the algae growth are identical.Exogenous NO can make up the algae growth degraded by low illumination.The influence of NO on the photosynthesis pigments content is consistent with that on algae density.The compound proteins constitute of Chl-a did not emerge marked change when NO were added,but the contents of photosynthesis pigments and their relative compose were affected.NO can improve the transfer efficiency of cell exploding energy,and enhance the photosynthesis speed,so the algae cell growths are quickened,and the algae biomass are increased.