| 氧四环素的微生物燃料电池处理及微生物群落 |
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| 引用本文： | 严伟富,肖勇,王淑华,丁蕊,赵峰. 氧四环素的微生物燃料电池处理及微生物群落[J]. 环境科学, 2018, 39(3): 1379-1385 |
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| 作者姓名： | 严伟富 肖勇 王淑华 丁蕊 赵峰 |
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| 作者单位： | 中国科学院城市环境研究所, 中国科学院城市污染物转化重点实验室, 厦门 361021;中国科学院大学, 北京 100049,中国科学院城市环境研究所, 中国科学院城市污染物转化重点实验室, 厦门 361021,中国科学院城市环境研究所, 中国科学院城市污染物转化重点实验室, 厦门 361021;中国科学院大学, 北京 100049,中国科学院城市环境研究所, 中国科学院城市污染物转化重点实验室, 厦门 361021;中国科学院大学, 北京 100049,中国科学院城市环境研究所, 中国科学院城市污染物转化重点实验室, 厦门 361021 |
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| 基金项目： | 国家自然科学基金项目（51478451，51208490）；中国科学院知识创新工程青年人才领域前沿项目（IUEQN201306） |
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| 摘 要： | 氧四环素(OTC)作为一种广谱性抗生素而被大量使用,其滥用不仅直接破坏生态系统,更容易引起微生物耐药性和抗性基因污染等问题.本研究利用微生物燃料电池(MFC)处理OTC,研究OTC在MFC不同运行时期的去除率变化情况,发现在运行150 d后,MFC对10 mg·L-1OTC的去除率在132 h达到99.0%.利用高通量测序技术分析并比较了原始接种猪粪与运行150 d后MFC阳极生物膜的微生物群落结构,发现厚壁菌门(Firmicutes)处于优势地位,但相比于原始接种猪粪,MFC生物膜上的变形菌门(Proteobacteria)的丰度从2.84%提高至8.92%~22.75%,此外,真细菌属(Eubacterium)的比例从几乎为0.00%显著提高至20.49%~49.00%.根据现有研究报道,Eubacterium spp.对多种氧杂环芳香族化合物具有一定的生物降解能力,本研究表明Eubacterium spp.可能是一类具有较强OTC降解能力的功能微生物.
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| 关 键 词： | 微生物燃料电池 氧四环素 抗生素抗性 微生物群落 高通量测序 |
| 收稿时间： | 2017-08-21 |
| 修稿时间： | 2017-09-02 |
Oxytetracycline Wastewater Treatment in Microbial Fuel Cells and the Analysis of Microbial Communities |
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| YAN Wei-fu,XIAO Yong,WANG Shu-hu,DING Rui and ZHAO Feng. Oxytetracycline Wastewater Treatment in Microbial Fuel Cells and the Analysis of Microbial Communities[J]. Chinese Journal of Environmental Science, 2018, 39(3): 1379-1385 |
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| Authors: | YAN Wei-fu XIAO Yong WANG Shu-hu DING Rui ZHAO Feng |
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| Affiliation: | Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China,Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China and Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China |
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| Abstract: | Oxytetracycline (OTC) as an important broad spectrum antibiotic has been widely used in animal husbandry. However, the abuse of OTC not only has a direct adverse impact on ecosystems, but also exacerbates the appearance of antibiotic resistance bacteria and antibiotic resistance genes. OTC-containing wastewater was treated using microbial fuel cells (MFCs), and the removal efficiency of OTC in MFCs in different operation periods was investigated. Result showed that the removal efficiency of 10 mg·L-1 OTC by MFCs within 132 h was up to 99.0% after 150 d of operation. The bacterial communities in raw pig mature and anodic biofilms were studied by high-throughput sequencing. This showed that Firmicutes were both dominant on phylum a level; However, compared to the raw pig mature, the abundance of Proteobacteria greatly increased from 2.84% to 8.92%-22.75% in the anodic biofilm. In addition, the abundance of Eubacterium spp. in the anodic biofilm increased obviously from nearly 0.00% to 20.49%-49.00%. It has been reported that some Eubacterium spp. were able to biodegrade oxygen heterocyclic aromatic compounds contained in OTC. Therefore, Eubacterium spp. is suggested as potential functional species in the biodegradation of OTC and/or its metabolites. This work proves the feasibility and effectiveness of removing OTC-containing wastewater by using MFCs. |
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| Keywords: | microbial fuel cell oxytetracycline(OTC) antibiotic resistance microbial community high-throughput sequencing |
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