产表面活性剂菌筛选及其对柴油降解影响研究

运用微生物培养方法,从饭店排污口污泥中筛选了7株产表面活性剂菌株。为了研究产表面活性剂菌在柴油降解过程中所产生的影响,分别将这些菌株与柴油降解菌(实验室提取的另一株假单胞菌)共同作用,其中一株(B_(26))不但可以将降解诱导期从6 d缩短至4 d,而且能将降解率由原来的71.1%提高至80.6%,具有较大的研究价值。实验着重对B_(26)的作用机理、柴油降解的影响因素等进行了分析研究。经形态学和生理生化实验鉴定,B_(26)属于芽孢菌属,其代谢过程中产生脂肽类表面活性剂,利用冷冻干燥法提取生物表面活性剂产量为1.57 g/L。

Isolation of biosurfactant producing microorganisms and influence on degradation of diesel oil

Seven biosurfactant producing strains, obtained from sewage of a restaurant by method of microbe cultivation, were respectively interacted with a diesel oil degradable bacteria (a kind of Pseudomonas strains obtained also in lab) to study the influences of biosurfactants on diesel oil degradation. The interaction showed that B₂₆, one of the seven strains, could not only reduce the degradation induction peoriod from 6 days to 4 days but also increase the degradation rate from 71.1% to 80.6%, which deserved further research. All tests were focused on analysis of mechanism and the affecting factors in diesel oil degradation. Based on the morphological, physiological and biochemical test results, B₂₆ was identified as Bacillus sp., in whose metabolism lipopeptide biosurfactant could be produced, and 1.57 g/L of them could be extracted using the freeze-drying method.