| 磷脂酶高产菌株的筛选、诱变及其在豆油脱胶中的应用 |
| |
| 引用本文： | 王金梅,姜芳燕,戴大章,李 春.磷脂酶高产菌株的筛选、诱变及其在豆油脱胶中的应用[J].环境科学研究,2010,23(7):948-952. |
| |
| 作者姓名： | 王金梅 姜芳燕 戴大章 李 春 |
| |
| 作者单位： | 1.石河子大学化学化工学院, 新疆兵团化工绿色过程重点实验室, 新疆 石河子 832000 |
| |
| 基金项目： | 国家水体污染控制与治理科技重大专项 |
| |
| 摘 要： | 采用以磷脂为唯一碳源的限制性培养基，从富油土样中筛选得到一株磷脂酶活性较高的菌株Achromobacter sp. BIT-56. 经紫外诱变处理后其磷脂酶活力比原始菌株提高了73.3%，进一步采用紫外-硫酸二乙酯(DES)复合诱变后其磷脂酶活力提高了95.6%. 利用该菌株生产的磷脂酶进行豆油脱胶研究，其适宜的脱胶条件:脱胶温度为40 ℃，pH为5.0，加酶量为100 U/kg，加水量为4%，脱胶时间为4 h，在该条件下脱胶油中磷脂的去除率高达75.6%.
|
| 关 键 词： | 磷脂酶 筛选 诱变 酶法脱胶 |
| 收稿时间： | 2010/1/19 0:00:00 |
| 修稿时间： | 2010/3/5 0:00:00 |
Screening and Mutation of a High-Yield Phospholipase Strain and Its Application in Soybean Oil Degumming |
| |
| WANG Jin-mei,JIANG Fang-yan,DAI Da-zhang and LI Chun.Screening and Mutation of a High-Yield Phospholipase Strain and Its Application in Soybean Oil Degumming[J].Research of Environmental Sciences,2010,23(7):948-952. |
| |
| Authors: | WANG Jin-mei JIANG Fang-yan DAI Da-zhang and LI Chun |
| |
| Institution: | 1.Key Laboratory for Green Processing of Chemical Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832000, China2.Department of Biological Engineering, School of Life Science, Beijing Institute of Technology, Beijing 100081, China |
| |
| Abstract: | Using phospholipid as the sole carbon source, Achromobacter sp. BIT-56 with a high-yield phospholipase was screened out from the oil-rich soil samples. Activity of the phospholipase produced by the best mutation strain could be improved by 73.3% through UV-induced mutation and 95.6% through UV-diethyl sulfate (DES) complex mutation as compared with the wild strain. When 100 U/kg of this phospholipase was added in the degumming of soybean oil and reacted at pH 5.0, 4% H2O, and 40 ℃ for 4 h, 75.6% of the phospholipid of the soybean oil could be removed. |
| |
| Keywords: | phospholipase screening mutation enzymatic degumming |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《环境科学研究》浏览原始摘要信息 |
| 点击此处可从《环境科学研究》下载免费的PDF全文 |
|
