不同形态锌离子对鲫鱼谷胱甘肽系统的影响

 研究了在室内模拟条件下,不同形态锌离子(Zn²⁺与Zn-EDTA)长期(40d)暴露对鲫鱼(Crucian curatus)肝脏谷胱甘肽系统的影响.结果表明,在试验剂量范围内,0.10mg/L的Zn²⁺暴露即能引起鲫鱼肝脏中锌显著积累,明显高于Zn-EDTA暴露试验中的积累量.鲫鱼肝脏中谷胱甘肽还原酶(GR)的活性,在Zn²⁺及Zn-EDTA浓度为0.05mg/L(低于渔业水质标准0.10mg/L)时就受到了显著抑制,Zn-EDTA较高浓度(>0.1mg/L)暴露对GR活性的抑制率更大.Zn²⁺对谷胱甘肽过氧化物酶(GPx)活性、氧化型谷胱甘肽(GSSG)、还原型谷胱甘肽(GSH)的含量及GSH/GSSG均有抑制作用,且存在良好的剂量-效应关系.

Effects of zinc ions of different patterns on glutathione system of Crucian curatus

Under indoor simulating condition, the influence of zinc ions of different patterns (Z2 and Zn-EDTA) long-term (40d) exposure on Crucian curatus glutathione system was studied. In the range of test dosage, exposure of 0.10mg/L Zn2 could cause marked zinc accumulation in liver of Crucian curatus, obviously higher than the amount of accumulation in the test of Zn-EDTA exposure. When the concentrations of Zn2 and Zn-EDTA were 0.05mg/L (below the fishery water quality standard 0.10mg/L), the glutathione reducase GR activity was inhibited markedly. Exposure to higher Zn-EDTA concentrations (>0.1mg/L) had greater inhibitation rate on GR activity. Zn2 had inhibition action on the activity of glutathione peroxisome (GPx), contents of oxidized glutathione (GSSG) and reduced glutathione (GSH) and GSH/GSSG, and existed good dosage-efect relation.